![ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] - Samples were spiked with high concentrations of Mouse Caspase-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75042_mouse-caspase-9-elisa-kit-colorimetric-13220241617344.jpg "ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] - Samples were spiked with high concentrations of Mouse Caspase-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | MuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Specificity | This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed. |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 5.31% |
| Intra-Assay | CV% < 4.39% |
| Spike Recovery | 87-106% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | CASP9 |
| Dilutions |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
| Publication using NBP2-75042 | Applications | Species |
|---|---|---|
| Xiaowei X, Qian X, Dingzhou Z Sirtuin-3 activates the mitochondrial unfolded protein response and reduces cerebral ischemia/reperfusion injury International journal of biological sciences 2023-08-21 [PMID: 37705748] (ELISA) | ELISA |
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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The Ins and Outs of Survivin By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ... Read full blog post. |
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Caspase-3, The Executioner of Apoptosis The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat... Read full blog post. |
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Caspase 9 - an important apoptosis marker Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i... Read full blog post. |
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| Gene Symbol | CASP9 |
![ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75042_mouse-caspase-9-elisa-kit-colorimetric-432024973640.jpg "ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] - Standard Curve Reference")
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![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.](https://images.novusbio.com/images/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0012.jpg "Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.")