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Human Caspase-8 ELISA Kit (Colorimetric)

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ELISA: Caspase-8 ELISA Kit [NBP1-83733] - Standard curve
ELISA: Caspase-8 ELISA Kit [NBP1-83733] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, cell lysate, serum
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.10 ng/mL

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Human Caspase-8 ELISA Kit (Colorimetric) Summary

Description
The human Caspase-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-8
Specificity
This kit detect both forms, the pro- as well as the active form of Caspase-8.
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.5%
Intra-Assay
CV% < 6.7%
Spike Recovery
89.5%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASP8

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
  3. anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
  4. Anti-rabbit-IgG-HRP
  5. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  6. Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
  7. Lysis Buffer 10x
  8. Sample Diluent
  9. Stop Solution (1M Phosphoric acid)
  10. Substrate Solution (tetramethyl-benzidine)
  11. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human Caspase-8 ELISA Kit (Colorimetric)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

View specific protocols for Caspase-8 ELISA Kit (NBP1-83733): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 ELISA Kit (NBP1-83733). (Showing 1 - 2 of 2 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
  2. Does NBP1-83733 recognize the cleaved enzyme (the active rpotein) or the total form of the protein?
    • The antibodies in this kit detect both forms, the pro- as well as the active form of Caspase-8.

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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.
VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.
Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.
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Bioinformatics

Gene Symbol CASP8