![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0004.jpg "Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 3B10 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant mouse caspase-8 (p20 subunit). |
| Specificity | Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | CASP8 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Caspase-8 Antibody (NBP2-80098)Find related products by research area.
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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ... Read full blog post. |
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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Caspase-3, The Executioner of Apoptosis The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat... Read full blog post. |
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VPS41 - An important regulator of lysosomal trafficking Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab... Read full blog post. |
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Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req... Read full blog post. |
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Caspase 8 - a key mediator of apoptosis Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a... Read full blog post. |
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| Gene Symbol | CASP8 |
![Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (3B10) (Prod. No. NBP2-80098)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Flow-Cytometry-NBP2-80098-img0001.jpg "Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (3B10) (Prod. No. NBP2-80098)")
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0002.jpg "Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.")
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0003.jpg "Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.")
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