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Caspase-1 Antibody (661228) [Unconjugated]

 (19 Publications)
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Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse ...read more
Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
661228
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6215-SP
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Caspase-1 Antibody (661228) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Specificity
Detects human Caspase-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CASP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB6215 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-1 Antibody (661228) [Unconjugated]

  • CASP1 nirs variant 1
  • CASP1
  • CASP-1
  • caspase 1, apoptosis-related cysteine peptidase
  • Caspase-1 subunit p10
  • Caspase-1 subunit p20
  • Caspase1
  • Caspase-1
  • EC 3.4.22.36
  • EC:3.4.22.36
  • ICE
  • IL-1 beta-converting enzyme
  • IL1BC
  • IL-1BC
  • IL1BCE
  • IL1B-convertase
  • interleukin 1, beta, convertase
  • interleukin 1-B converting enzyme
  • Interleukin-1 beta convertase
  • Interleukin-1 beta-converting enzyme
  • p45

Background

Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-1 Antibody (MAB6215)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 3 applications: IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(12)
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Human
(9)
Mouse
(5)
Rat
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB6215 Applications Species
H Li, X Zhu, J Xu, L Li, W Kan, H Bao, J Xu, W Wang, Y Yang, P Chen, Y Zou, Y Feng, J Yang, J Du, G Wang The FXR mediated anti-depression effect of CDCA underpinned its therapeutic potentiation for MDD International immunopharmacology, 2022-12-28;115(0):109626. 2022-12-28 [PMID: 36584576] (Western Blot, Mouse) Western Blot Mouse
Elena Díaz-García, Kapil Nanwani-Nanwani, Sara García-Tovar, Enrique Alfaro, Eduardo López-Collazo, Manuel Quintana-Díaz et al. NLRP3 Inflammasome Overactivation in Patients with Aneurysmal Subarachnoid Hemorrhage Translational Stroke Research 6/1/2023 [PMID: 35819747]
Karki R, Lee S, Mall R et al. ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection Science immunology 5/19/2022 [PMID: 35587515]
YA Kung, HJ Chiang, ML Li, YN Gong, HP Chiu, CT Hung, PN Huang, SY Huang, PY Wang, TA Hsu, G Brewer, SR Shih Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis MBio, 2022-01-18;0(0):e0271721. 2022-01-18 [PMID: 35038927] (Western Blot, Human) Western Blot Human
S Lee, R Karki, Y Wang, LN Nguyen, RC Kalathur, TD Kanneganti AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence Nature, 2021-09-01;0(0):. 2021-09-01 [PMID: 34471287] (Western Blot, Human, Mouse, Transgenic Mouse) Western Blot Human, Mouse, Transgenic Mouse
S Lee, A Ishitsuka, T Kuroki, YH Lin, A Shibuya, T Hongu, Y Funakoshi, Y Kanaho, K Nagata, A Kawaguchi Arf6 exacerbates allergic asthma through cell-to-cell transmission of ASC inflammasomes JCI Insight, 2021-08-23;6(16):. 2021-08-23 [PMID: 34423792] (Western Blot, Mouse) Western Blot Mouse
Scott A Read, Brian S Gloss, Christopher Liddle, Jacob George, Golo Ahlenstiel Interferon-lambda 3 Exacerbates the Inflammatory Response to Microbial Ligands: Implications for SARS-CoV-2 Pathogenesis Journal of Inflammation Research 4/1/2021 [PMID: 33833547]
N Posta, É Cs?sz, M Oros, D Peth?, L Potor, G Kalló, Z Hendrik, KÉ Sikura, G Méhes, C Tóth, J Posta, G Balla, J Balla Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction Lab. Invest., 2020-02-13;0(0):. 2020-02-13 [PMID: 32054994] (Western Blot, Human) Western Blot Human
Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation Autophagy, 2020-02-16;0(0):1-24. 2020-02-16 [PMID: 32019420] (Western Blot, Human) Western Blot Human
S Pires, D Parker IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms Eur. J. Immunol., 2018-08-19;0(0):. 2018-08-19 [PMID: 30051912] (Western Blot, Mouse) Western Blot Mouse
Show All 19 Publications.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-1.

Check out the latest blog posts on Caspase-1.
The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements ...  Read full blog post.
NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.
Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens. ...  Read full blog post.
Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.
Caspase 1 - activating innate immune responses following infection or injury
Caspase-1 is an enzyme involved in the conversion of interleukin-1 into its active secreted form. Interleukin-1 mediates inflammatory responses during infection and disease. Caspase-1 is recruited to and activated by the inflammasome complex (1). Un...  Read full blog post.
Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.
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Bioinformatics

Gene Symbol CASP1
Uniprot