![Western Blot: Casein Antibody [H00001446-D01P] - Expression of adipose-specific genes and adipose-enhanced genes. Semi-quantitative RT-PCR and/or Western blot analysis (WB) of CSN1S1, SLC19A3, and LVRN (AQPEP) in selected tissues: subcutaneous adipose tissue (Ad), brain (Br), heart (He), kidney (Ki), lung (Lu), liver (Li), and skeletal muscle (Mu). Target sizes for RT-PCR: 213?bp (CSN1S1), 170?bp (SLC19A3) and 193?bp (LVRN (AQPEP)). Expected sizes for full-length proteins were 21.7?kDa and 55.7?kDa for CSN1S1 and SLC19A3, respectively. Cyclophilin (CYC) (325?bp) and GAPDH (38?kDa) were used as a reference gene and protein, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39582-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Casein-Antibody-Western-Blot-H00001446-D01P-img0005.jpg "Western Blot: Casein Antibody [H00001446-D01P] - Expression of adipose-specific genes and adipose-enhanced genes. Semi-quantitative RT-PCR and/or Western blot analysis (WB) of CSN1S1, SLC19A3, and LVRN (AQPEP) in selected tissues: subcutaneous adipose tissue (Ad), brain (Br), heart (He), kidney (Ki), lung (Lu), liver (Li), and skeletal muscle (Mu). Target sizes for RT-PCR: 213?bp (CSN1S1), 170?bp (SLC19A3) and 193?bp (LVRN (AQPEP)). Expected sizes for full-length proteins were 21.7?kDa and 55.7?kDa for CSN1S1 and SLC19A3, respectively. Cyclophilin (CYC) (325?bp) and GAPDH (38?kDa) were used as a reference gene and protein, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-39582-8) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody reactive against mammalian transfected lysate. |
| Immunogen | CSN1S1 (NP_001881.1, 1 a.a. - 185 a.a.) full-length human protein. MRLLILTCLVAVALARPKLPLRYPERLQNPSESSEPIPLESREEYMNGMNRQRNILREKQTDEIKDTRNESTQNCVVAEPEKMESSISSSSEEMSLSKCAEQFCRLNEYNQLQLQAAHAQEQIRRMNENSHVQVPFQQLNQLAAYPYAVWYYPQIMQYVPFPPFSDISNPTAHENYEKNNVMLQW |
| Specificity | CSN1S1, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CSN1S1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | It has been used for ELISA and WB. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using H00001446-D01P | Applications | Species |
|---|---|---|
| Ahn J, Wu H, Lee K. Integrative Analysis Revealing Human Adipose-Specific Genes and Consolidating Obesity Loci Sci Rep 2019-02-28 [PMID: 30816281] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Casein Antibody (H00001446-D01P)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CSN1S1 |
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| Uniprot |
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![Western Blot: Casein Antibody [H00001446-D01P] - Analysis of CSN1S1 expression in mouse liver.](http://images.novusbio.com/fullsize/Casein-Antibody-Western-Blot-H00001446-D01P-img0003.jpg "Western Blot: Casein Antibody [H00001446-D01P] - Analysis of CSN1S1 expression in mouse liver.")
![Western Blot: Casein Antibody [H00001446-D01P] - Analysis of CSN1S1 expression in transfected 293T cell line by CSN1S1 polyclonal antibody.Lane 1: CSN1S1 transfected lysate(21.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Casein-Antibody-Western-Blot-H00001446-D01P-img0004.jpg "Western Blot: Casein Antibody [H00001446-D01P] - Analysis of CSN1S1 expression in transfected 293T cell line by CSN1S1 polyclonal antibody.Lane 1: CSN1S1 transfected lysate(21.70 KDa).Lane 2: Non-transfected lysate.")
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![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
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![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
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![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")