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Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

 (16 Publications)
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Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2344-SP
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Carbonic Anhydrase IX/CA9 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9
Gln32-Asp390
Accession # Q8VHB5
Specificity
Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than
1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Car9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2344 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  3. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (AF2344)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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ICC
(1)
IHC
(5)
IHC-Fr
(2)
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(2)
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(4)
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(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF2344 Applications Species
I Uribesalgo, D Hoffmann, Y Zhang, A Kavirayani, J Lazovic, J Berta, M Novatchkov, TP Pai, RA Wimmer, V László, D Schramek, R Karim, L Tortola, S Deswal, L Haas, J Zuber, M Sz?cs, K Kuba, B Dome, Y Cao, BJ Haubner, JM Penninger Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy EMBO Mol Med, 2019-06-24;0(0):e9266. 2019-06-24 [PMID: 31267692] (IHC, Transgenic Mouse) IHC Transgenic Mouse
E Johansson, ES Grassi, V Pantazopou, B Tong, D Lindgren, TJ Berg, EJ Pietras, H Axelson, A Pietras CD44 Interacts with HIF-2? to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells Cell Rep, 2017-08-15;20(7):1641-1653. 2017-08-15 [PMID: 28813675] (IHC, Mouse) IHC Mouse
M Swayampaku, PC McDonald, M Vallejo, E Coyaud, SC Chafe, A Westerback, G Venkateswa, J Shankar, G Gao, EMN Laurent, Y Lou, KL Bennewith, CT Supuran, IR Nabi, B Raught, S Dedhar The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion Oncogene, 2017-07-10;36(45):6244-6261. 2017-07-10 [PMID: 28692057] (Western Blot, Human) Western Blot Human
Yi-Feng Gu, Shannon Cohn, Alana Christie, Tiffani McKenzie, Nicholas Wolff, Quyen N. Do et al. Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade Cancer Discovery 8/1/2017 [PMID: 28473526]
Gabi U Dachs Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice Hypoxia (Auckl), 2016-04-08;4(0):41-52. 2016-04-08 [PMID: 27800507] (Western Blot, Mouse) Western Blot Mouse
Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice Sci Rep, 2016-08-05;6(0):30739. 2016-08-05 [PMID: 27491826] (IHC-P, Mouse) IHC-P Mouse
Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis. PLoS ONE, 2014-09-15;9(9):e106976. 2014-09-15 [PMID: 25222747] (IHC-P, Mouse) IHC-P Mouse
Brian Meehan, Delphine Garnier, Alexander Dombrovsky, Karrie Lau, Esterina D’Asti, Nathalie Magnus et al. Ageing-related responses to antiangiogenic effects of sunitinib in atherosclerosis-prone mice Mechanisms of Ageing and Development 9/1/2014 [PMID: 25068886]
Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation. PLoS ONE, 2014-05-01;9(5):e96007. 2014-05-01 [PMID: 24789143] (IHC, Mouse) IHC Mouse
John G. Facciponte, Stefano Ugel, Francesco De Sanctis, Chunsheng Li, Liping Wang, Gautham Nair et al. Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature Journal of Clinical Investigation 4/1/2014 [PMID: 24642465]
Show All 16 Publications.

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Secondary Antibodies

 

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Additional Carbonic Anhydrase IX/CA9 Products

Blogs on Carbonic Anhydrase IX/CA9.

Check out the latest blog posts on Carbonic Anhydrase IX/CA9.
Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol Car9
Uniprot