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Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

 (16 Publications)
 (1 Review)
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Western blot shows lysates of U-87 MG human glioblastoma/ astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Carbonic Anhydrase IX/CA9 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of U-87 MG human glioblastoma/ astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Carbonic Anhydrase IX/CA9 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188) at 3 ...read more
Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
U87-MG cells were stained with Goat Anti-Human Carbonic Anhydrase IX/CA9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2188, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2188
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Catalog# & Formulation Size Price
Carrier Free
AF2188-SP
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Carbonic Anhydrase IX/CA9 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CA9 is observed and less than 1% cross-reactivity with recombinant human CA1, 3, 4, 8, 10, 12, and 14 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CA9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2188 in the following applications:
Publications
Read Publications using
AF2188 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody [Unconjugated]

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). Recombinant human CA9 corresponds to the extracellular portion of human CA9.

  1. Pastorek, J. et al. (1994) Oncogene 9: 2877.
  2. Opavsky, R. et al. (1996) Genomics 33: 480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (AF2188)(16)

We have publications tested in 2 confirmed species: Human, Xenograft.

We have publications tested in 4 applications: CyTof, IHC, IHC-P, Western Blot.


Filter By Application
CyTof
(1)
IHC
(2)
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(1)
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(3)
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(5)
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(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF2188 Applications Species
Jana Raja Fischer, Hartland Warren Jackson, Natalie de Souza, Zsuzsanna Varga, Peter Schraml, Holger Moch et al. Multiplex imaging of breast cancer lymph node metastases identifies prognostic single-cell populations independent of clinical classifiers Cell Reports Medicine 2023-03-21 [PMID: 36921599]
Laura De Vargas Roditi, Andrea Jacobs, Jan H. Rueschoff, Pete Bankhead, Stéphane Chevrier, Hartland W. Jackson et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer Cell Reports Medicine 2022-04-19 [PMID: 35492239]
Eleanor R. Burgess, Rebekah L. I. Crake, Elisabeth Phillips, Helen R. Morrin, Janice A. Royds, Tania L. Slatter et al. Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient Survival Frontiers in Oncology 2022-03-28 [PMID: 35419292]
Anne E.G. Lenferink, Paul C. McDonald, Christiane Cantin, Suzanne Grothé, Mylene Gosselin, Jason Baardsnes et al. Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX mAbs 2021-11-22 [PMID: 34806527]
SC Chafe, FS Vizeacouma, G Venkateswa, O Nemirovsky, S Awrey, WS Brown, PC McDonald, F Carta, A Metcalfe, JM Karasinska, L Huang, SK Muthuswamy, DF Schaeffer, DJ Renouf, CT Supuran, FJ Vizeacouma, S Dedhar Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors Science Advances, 2021-08-27;7(35):. 2021-08-27 [PMID: 34452919] (Western Blot, Human) Western Blot Human
S Lefort, A El-Naggar, S Tan, S Colborne, GL Negri, D Pellacani, M Hirst, B Gusterson, GB Morin, PH Sorensen, CJ Eaves De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancer Cell Death and Differentiation, 2021-07-22;0(0):. 2021-07-22 [PMID: 34294889] (IHC, Human) IHC Human
Rory M. Bade, Jennifer L. Schehr, Hamid Emamekhoo, Benjamin K. Gibbs, Tamara S. Rodems, Matthew C. Mannino et al. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma Molecular Oncology 2021-09-01 [PMID: 33604999]
Gabi U. Dachs, Jamish Gandhi, Christina Wohlrab, Anitra C. Carr, Helen R. Morrin, Juliet M. Pullar et al. Vitamin C Administration by Intravenous Infusion Increases Tumor Ascorbate Content in Patients With Colon Cancer: A Clinical Intervention Study Frontiers in Oncology 2021-01-11 [PMID: 33505915]
EE Hedlund, PC McDonald, O Nemirovsky, S Awrey, LDE Jensen, S Dedhar Harnessing Induced Essentiality: Targeting Carbonic Anhydrase IX and Angiogenesis Reduces Lung Metastasis of Triple Negative Breast Cancer Xenografts Cancers (Basel), 2019-07-17;11(7):. 2019-07-17 [PMID: 31319613] (IHC, IHC-P, Xenograft) IHC, IHC-P Xenograft
Johanna Wagner, Maria Anna Rapsomaniki, Stéphane Chevrier, Tobias Anzeneder, Claus Langwieder, August Dykgers et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer Cell 2019-05-16 [PMID: 30982598]
Show All 16 Publications.

Review for Carbonic Anhydrase IX/CA9 Antibody (AF2188) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2188:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Carbonic Anhydrase IX/CA9 AF2188
Enlarge 		5
reviewed by:
Santhosh Sivajothi
 IHC-P Human 05/18/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRenal Cell Carcinoma tissues
SpeciesHuman
LotIDL0217051

Comments

CommentsHeat mediated antigen retrieval performed in citrate buffer (pH 6) for 20 minutes.

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FAQs for Carbonic Anhydrase IX/CA9 Antibody (AF2188) (0)

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Secondary Antibodies

 

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Additional Carbonic Anhydrase IX/CA9 Products

Blogs on Carbonic Anhydrase IX/CA9.

Check out the latest blog posts on Carbonic Anhydrase IX/CA9.
Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.
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Immunohistochemistry-Paraffin Carbonic Anhydrase IX/CA9 AF2188
Enlarge
 Santhosh Sivajothi
05/18/2018
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol CA9
Uniprot