![ELISA: Carbonic Anhydrase I/CA1 Antibody [Biotin] [NBP1-77740] - ELISA Results of Goat Carbonic Anhydrase I/CA1/CA1 antibody [Biotin] tested against purified Carbonic Anhydrase I/CA1 Biotin. Each well was coated in duplicate with 1.0 ug of Carbonic Anhydrase I/CA1. The working dilution is 1:6900. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-[Biotin]-ELISA-NBP1-77740-img0001.jpg "ELISA: Carbonic Anhydrase I/CA1 Antibody [Biotin] [NBP1-77740] - ELISA Results of Goat Carbonic Anhydrase I/CA1/CA1 antibody [Biotin] tested against purified Carbonic Anhydrase I/CA1 Biotin. Each well was coated in duplicate with 1.0 ug of Carbonic Anhydrase I/CA1. The working dilution is 1:6900. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated, and TMB substrate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NB600-942-0.1mg | |||
| Unconjugated | NB120-6619-0.1mg | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This antibody (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes] |
| Immunogen | Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915) |
| Localization | Cytoplasmic |
| Specificity | Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CA1 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher. |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
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