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carabin Antibody

Images

 
Western Blot: carabin Antibody [NBP2-76349] - Staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Flow Cytometry: carabin Antibody [NBP2-76349] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ...read more
Western Blot: carabin Antibody [NBP2-76349] - Staining of Jurkat with antibody at 0.5 ug/mL (A), PBM with antibody at 0.3 ug/mL (B) and negative control A431 (C) cell lysate (35 ug protein in RIPA buffer). Detected by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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carabin Antibody Summary

Immunogen
Peptide with sequence C-PRRKPQTRGKTFH corresponding to internal region according to NP_940919.1.
Predicted Species
Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TBC1D10C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Peptide ELISA Detection limit 1:28000
  • Western Blot
Application Notes
WB: Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for carabin Antibody

  • carabin
  • FLJ00332
  • MGC46488
  • TBC1 domain family member 10C
  • TBC1 domain family, member 10C

Background

Carabin is an endogenous inhibitor of calcineurin (see MIM 114105) that also inhibits the Ras (see MIM 190020) signaling pathway through its intrinsic Ras GTPase-activating protein activity (Pan et al., 2007 [PubMed 17230191]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBC1D10C