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Canine TNF-alpha DuoSet ELISA, 15 Plate

 (17 Publications)
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Summary
Reactivity CaSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Canine TNF-alpha DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TNF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY1507.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine TNF-alpha DuoSet ELISA, 15 Plate

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Canine TNF-alpha
DuoSet ELISA, 15 Plat
DY1507
Canine TNF-alpha DuoSet ELISA, 15 Plate
Species: Ca
Applications: ELISA

Publications for TNF-alpha (DY1507)(17)

We have publications tested in 1 confirmed species: Canine.


Filter By Application
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Filter By Species
Canine
(17)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using DY1507 Applications Species
Chow, L;Wheat, W;Ramirez, D;Impastato, R;Dow, S; Direct comparison of canine and human immune responses using transcriptomic and functional analyses Scientific reports 2024-01-26 [PMID: 38272935] (Canine) Canine
Costa, SF;Soares, MF;Poleto Bragato, J;Dos Santos, MO;Rebech, GT;de Freitas, JH;de Lima, VMF; MicroRNA-194 regulates parasitic load and IL-1?-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis PLoS neglected tropical diseases 2024-01-01 [PMID: 38241360] (Canine) Canine
GT Rebech, JP Bragato, SF Costa, JH de Freitas, MO Dos Santos, MF Soares, FR Eugênio, PSP Dos Santos, VMF de Lima miR-148a regulation interferes in inflammatory cytokine and parasitic load in canine leishmaniasis PloS Neglected Tropical Diseases, 2023-01-31;17(1):e0011039. 2023-01-31 [PMID: 36719867] (Canine) Canine
JP Bragato, GT Rebech, JH Freitas, MOD Santos, SF Costa, FR Eugênio, PSPD Santos, VMF de Lima miRNA-21 regulates CD69 and IL-10 expression in canine leishmaniasis PLoS ONE, 2022-03-24;17(3):e0265192. 2022-03-24 [PMID: 35324917] (Canine) Canine
JK Abbott, SK Chan, M MacBeth, JL Crooks, C Hancock, V Knight, EW Gelfand Fluctuations in quality of life and immune responses during intravenous immunoglobulin infusion cycles PLoS ONE, 2022-03-22;17(3):e0265852. 2022-03-22 [PMID: 35316278] (Canine) Canine
MT Verde, S Villanueva, A Loste, D Marteles, D Pereboom, T Conde, A Fernández Comparison of circulating CD4+, CD8+ lymphocytes and cytokine profiles between dogs with atopic dermatitis and healthy dogs Research in Veterinary Science, 2022-01-29;145(0):13-20. 2022-01-29 [PMID: 35134678] (Canine) Canine
RM Amorim, KC Clark, NJ Walker, P Kumar, K Herout, DL Borjesson, A Wang Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease Stem Cell Res Ther, 2020-07-22;11(1):304. 2020-07-22 [PMID: 32698861] (Canine) Canine
W Wheat, L Chow, A Kuzmik, S Soontarara, J Kurihara, M Lappin, S Dow Local immune and microbiological responses to mucosal administration of a Liposome-TLR agonist immunotherapeutic in dogs BMC Vet. Res., 2019-09-13;15(1):330. 2019-09-13 [PMID: 31519215] (Canine) Canine
KL Oliveira S, V Marin Chik, G Luvizotto, AA Correa Lea, BF de Almeida, F De Rezende, PSP Dos Santos, G Fabrino Ma, VMF De Lima PD-1 and PD-L1 regulate cellular immunity in canine visceral leishmaniasis Comp. Immunol. Microbiol. Infect. Dis., 2018-12-14;62(0):76-87. 2018-12-14 [PMID: 30711051] (Canine) Canine
H Yang, W Kim, J Bae, H Kim, S Kim, J Choi, J Park, DI Jung, H Koh, D Yu Effects of irradiation and leukoreduction on down regulation of CXCL-8 and storage lesion in stored canine whole blood J. Vet. Sci., 2019-01-31;0(0):. 2019-01-31 [PMID: 30541183] (Canine) Canine
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha (DY1507). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Bioinformatics

Gene Symbol TNF