Novus Biologicals products are now on bio-techne.com

CAMP/LL37/FALL39/Cathelicidin Overexpression Lysate

Images

 
Western Blot: Cathelicidin Overexpression Lysate (Adult Normal) [NBL1-08670] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cathelicidin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

CAMP/LL37/FALL39/Cathelicidin Overexpression Lysate Summary

Description

CAMP/LL37/FALL39/Cathelicidin Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208872

Accession#: NM_004345

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CAMP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
19.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CAMP/LL37/FALL39/Cathelicidin Overexpression Lysate

  • 18 kDa cationic antimicrobial protein
  • CAMP
  • CAMP/LL37
  • CAP18
  • CAP18CRAMP
  • cathelicidin antimicrobial peptide
  • CRAMP
  • FALL39
  • FALL-39
  • FALL39CAP-18
  • hCAP-18
  • LL37

Background

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
AF2758
Species: Hu
Applications: IHC, WB
NBP2-34906
Species: Hu
Applications: BA, PAGE
NBP2-24729
Species: Ca, Eq, Hu, Pm, Mu, Pm, Rt
Applications: B/N, CyTOF-ready, DB, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, Simple Western, WB
NBP3-03780
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NLS1878
Species: Ba, Hu, Mu, Rt
Applications: EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
H00001594-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
DY870-05
Species: Hu
Applications: ELISA
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
7468-BP
Species: Hu
Applications: BA
NBP1-85495
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DY413
Species: Mu
Applications: ELISA

Publications for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670) (0)

There are no publications for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670) (0)

There are no reviews for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CAMP/LL37/FALL39/Cathelicidin Products

Research Areas for CAMP/LL37/FALL39/Cathelicidin Lysate (NBL1-08670)

Find related products by research area.

Blogs on CAMP/LL37/FALL39/Cathelicidin

There are no specific blogs for CAMP/LL37/FALL39/Cathelicidin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CAMP/LL37/FALL39/Cathelicidin Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol CAMP