![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer).](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0033.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | OSX12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | A synthetic peptide from human CAMP/Cathelicidin conjugated to an immunogenic carrier protein has been used as the antigen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CAMP |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The optimal concentration should be determined by the end user. Use in immoprecipitation reported in scientific literature (PMID 26508735) |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Sterilized water |
| Preservative | No Preservative |
| Purity | Ammonium sulfate precipitation |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
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| Publication using NBP1-46781 | Applications | Species |
|---|---|---|
| Svensson D, Wilk L, Morgelin M et al. LL-37-induced host cell cytotoxicity depends on cellular expression of the globular C1q receptor (p33). Biochem J 2016-01-01 [PMID: 26508735] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells.](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0027.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells.")
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0028.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0029.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0030.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0031.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining on ethanol-fixed human spermatosoid using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells](http://images.novusbio.com/fullsize/CAMP-LL37-FALL39-Cathelicidin-Antibody-OSX12-Immunocytochemistry-Immunofluorescence-NBP1-46781-img0032.jpg "Immunocytochemistry/Immunofluorescence: CAMP/Cathelicidin Antibody (OSX12) [NBP1-46781] - Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells")
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