![Immunohistochemistry: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32614) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32614_rabbit-ca125-muc16-mab-pd01-13-29820241358558.jpg "Immunohistochemistry: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32614) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | PD01-13 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide. (Uniprot: Q8WXI7) |
| Localization | Cell membrane, secreted, extracellular space. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MUC16 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 1519 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CA125/MUC16 Antibody (NBP3-32614)Find related products by research area.
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| Gene Symbol | MUC16 |
![Immunocytochemistry/ Immunofluorescence: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunocytochemistry analysis of SKOV-3 cells labeling CA125/MUC16 with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32614_rabbit-ca125-muc16-mab-pd01-13-29202410484871.jpg "Immunocytochemistry/ Immunofluorescence: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunocytochemistry analysis of SKOV-3 cells labeling CA125/MUC16 with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
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