BubR1 Antibody (8G1) [FITC]

Images

 
There are currently no images for BubR1 Antibody (NB100-353F).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
Clone
8G1
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

BubR1 Antibody (8G1) [FITC] Summary

Immunogen
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
Localization
Nuclear
Specificity
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for BubR1 Antibody (8G1) [FITC]

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

BubR1 (budding uninhibited by benzimidazole-related 1 or mitotic checkpoint serine/threonine-protein kinase BUB1 beta) is an essential component of mitotic checkpoint/control complex which is required for normal mitotic progression and any defects in this complex may result in chromosomal instability/aberrant mitosis. This evolutionarily conserved and crucial system of self-control in cell cycle warrants high quality of chromosomal segregation by postponing manifestation of anaphase in all chromosomes which are incorrectly bi-oriented to mitotic spindles. One of its checkpoint functions is to prevent the activity of anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity; and the other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. BubR1 is required for kinetochore localization of CENPE and it negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. BubR1 is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest and thus it behaves as a tumor suppressior. Defects in BubR1 have been associated with tumor formation and are the underlying cause of premature chromatid separation trait (PCS) as well as mosaic variegated aneuploidy syndrome type 1 (MVA1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-563
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF4185
Species: Hu
Applications: ICC
H00000699-D01P
Species: Hu, Mu
Applications: ICC/IF, PLA, WB
NB100-59828
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-88517
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF4885
Species: Mu
Applications: IP, WB
NBP1-81765
Species: Hu
Applications: IHC, IHC-P
NBP1-82875
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32775
Species: Hu, Mar, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
MAB6028
Species: Hu
Applications: WB
NBP2-85249
Species: Hu, Mu
Applications: WB
NB100-294
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP1-48291
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NBP3-19989
Species: Hu, Mu, Rt
Applications: WB
NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56479
Species: Bv, Ca, Hu, Mu, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF6000
Species: Hu, Mu
Applications: IHC, Simple Western, WB
NB100-353F
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready

Publications for BubR1 Antibody (NB100-353F) (0)

There are no publications for BubR1 Antibody (NB100-353F).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BubR1 Antibody (NB100-353F) (0)

There are no reviews for BubR1 Antibody (NB100-353F). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BubR1 Antibody (NB100-353F) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BubR1 Antibody (8G1) [FITC] and receive a gift card or discount.

Bioinformatics

Gene Symbol BUB1B