BubR1 Antibody (8G1) [FITC] Summary
Immunogen |
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566] |
Localization |
Nuclear |
Specificity |
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1. |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
BUB1B |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
- Immunoprecipitation
- Western Blot
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Purity |
Protein G purified |
Alternate Names for BubR1 Antibody (8G1) [FITC]
Background
BubR1 (budding uninhibited by benzimidazole-related 1 or mitotic checkpoint serine/threonine-protein kinase BUB1 beta) is an essential component of mitotic checkpoint/control complex which is required for normal mitotic progression and any defects in this complex may result in chromosomal instability/aberrant mitosis. This evolutionarily conserved and crucial system of self-control in cell cycle warrants high quality of chromosomal segregation by postponing manifestation of anaphase in all chromosomes which are incorrectly bi-oriented to mitotic spindles. One of its checkpoint functions is to prevent the activity of anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity; and the other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. BubR1 is required for kinetochore localization of CENPE and it negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. BubR1 is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest and thus it behaves as a tumor suppressior. Defects in BubR1 have been associated with tumor formation and are the underlying cause of premature chromatid separation trait (PCS) as well as mosaic variegated aneuploidy syndrome type 1 (MVA1).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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