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Bovine TNF-alpha DuoSet ELISA, 15 Plate

 (19 Publications)
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Summary
Reactivity BvSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Bovine TNF-alpha DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TNF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY2279.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bovine TNF-alpha DuoSet ELISA, 15 Plate

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Bovine TNF-alpha
DuoSet ELISA, 15 Plat
DY2279
Bovine TNF-alpha DuoSet ELISA, 15 Plate
Species: Bv
Applications: ELISA

Publications for TNF-alpha (DY2279)(19)

We have publications tested in 1 confirmed species: Bovine.


Filter By Application
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Filter By Species
Bovine
(19)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using DY2279 Applications Species
Varma, VP;Bankala, R;Kumar, A;Gawai, S;Faisal, SM; Differential modulation of innate immune response by lipopolysaccharide of Leptospira Open biology 2023-11-01 [PMID: 37935355] (Bovine) Bovine
A Hod, JR Daddam, G Kra, H Kamer, Y Portnick, U Moallem, M Zachut Glucose-6-Phosphate Dehydrogenase Activity in Milk May Serve as a Non-Invasive Metabolic Biomarker of Energy Balance in Postpartum Dairy Cows Metabolites, 2023-02-20;13(2):. 2023-02-20 [PMID: 36837930] (Bovine) Bovine
J Wu, F Bai, W Mao, B Liu, X Yang, J Zhang, T Li, G Borjigin, J Cao Anti-inflammatory effects of the prostaglandin D2/prostaglandin DP1 receptor and lipocalin-type prostaglandin D2 synthase/prostaglandin D2 pathways in bacteria-induced bovine endometrial tissue Veterinary research, 2022-11-26;53(1):98. 2022-11-26 [PMID: 36435808] (Bovine) Bovine
Y Cai, MS Gilbert, WJJ Gerrits, G Folkerts, S Braber Galacto-oligosaccharides alleviate lung inflammation by inhibiting NLRP3 inflammasome activation in vivo and in vitro Journal of advanced research, 2021-11-01;39(0):305-318. 2021-11-01 [PMID: 35777914] (Bovine) Bovine
PC Duque-Madr, J Velasco-Bo, A Ceballos-M, C López, JU Carmona Intramammary treatment using allogeneic pure platelet-rich plasma in cows with subclinical mastitis caused by Gram-positive bacteria Scientific Reports, 2021-12-09;11(1):23737. 2021-12-09 [PMID: 34887474] (Bovine) Bovine
V Filor, M Petry, J Mei beta ner, M Kietzmann Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis Bmc Veterinary Research, 2021-03-16;17(1):120. 2021-03-16 [PMID: 33726750] (Bovine) Bovine
T Xu, X Wu, X Lu, Y Liang, Y Mao, JJ Loor, Z Yang Metformin activated AMPK signaling contributes to the alleviation of LPS-induced inflammatory responses in bovine mammary epithelial cells Bmc Veterinary Research, 2021-03-01;17(1):97. 2021-03-01 [PMID: 33648513] (Bovine) Bovine
W Wheat, L Chow, V Rozo, J Herman, K Still Broo, A Colbath, R Hunter, S Dow Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant PLoS ONE, 2020-06-25;15(6):e0235422. 2020-06-25 [PMID: 32584899] (Bovine) Bovine
T Ohtaki, K Ogata, H Kajikawa, T Sumiyoshi, S Asano, S Tsumagari, T Horikita Effect of high-concentrate corn grain diet-induced elevated ruminal lipopolysaccharide levels on dairy cow liver function J. Vet. Med. Sci., 2020-05-27;0(0):. 2020-05-27 [PMID: 32461536] (Bovine) Bovine
T Li, L Hai, B Liu, W Mao, K Liu, Yuan Shen, Q Li, Y Guo, Y Jia, H Bao, J Cao TLR2/4 promotes PGE2 production to increase tissue damage in Escherichia coli-infected bovine endometrial explants via MyD88/p38 MAPK pathway Theriogenology, 2020-04-07;152(0):129-138. 2020-04-07 [PMID: 32408026] (Bovine) Bovine
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha (DY2279). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Additional TNF-alpha Products

Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

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Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.
Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.
Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.
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Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol TNF