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Betacellulin/BTC Antibody (197417)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
197417
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB10251-SP
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Betacellulin/BTC Antibody (197417) Summary

Immunogen
E. coli-derived recombinant mouse Betacellulin
Asp32-Gln118
Accession # Q05928
Specificity
Detects mouse Betacellulin in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) Epigen, rhAR, rhBetacellulin, rhEGF, rhEpiregulin, rhNRG-2, rhNRG1-beta 1, or rhTGF-alpha .
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
BTC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Mouse Betacellulin/BTC Antibody (Catalog # MAB10251)
ELISA Detection: Mouse Betacellulin/BTC Biotinylated Antibody (Catalog # BAF1025)
Standard: Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Betacellulin/BTC Antibody (197417)

  • Betacellulin
  • BTC
  • probetacellulin

Background

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha , amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

  1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
  2. Shing, Y. et al. (1993) Science 259:1604.
  3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
  4. Pathak, B.G. et al. (1995) Genomics 28:116.
  5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
  6. Li, L. et al. (2001) Endocrinology 142:5379.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BTC
Uniprot