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beta Tubulin Antibody (1B12)

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Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061] - Western blot analysis of whole tissue or cell lysates using mouse mAb to Beta-tubulin, NBP2-50061, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50 kDa corresponds to the Beta-tubulin proteins.
Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061] - Western blot analysis of whole tissue or cell lysates using mouse mAb to Beta-tubulin, NBP2-50061, dilution 1:10,000 in green: [1] protein standard (red), [2] ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - HeLa cells stained with NBP2-50061 in green, and chicken antibody to Lamin A+C in red (NBP2-25152), and DNA in blue. NBP2-50061 ...read more
Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - Immunofluorescent analysis of HeLa cells stained with NBP2-50061, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, Single-Cell Western
Clone
1B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50061AF350
Alexa Fluor 405
NBP2-50061AF405
Alexa Fluor 488
NBP2-50061AF488
Alexa Fluor 532
NBP2-50061AF532
Alexa Fluor 594
NBP2-50061AF594
Alexa Fluor 647
NBP2-50061AF647
Alexa Fluor 700
NBP2-50061AF700
Alexa Fluor 750
NBP2-50061AF750
Biotin
NBP2-50061B
CoraFluor 1
NBP2-50061CL1
DyLight 350
NBP2-50061UV
DyLight 405
NBP2-50061V
DyLight 488
NBP2-50061G
DyLight 550
NBP2-50061R
DyLight 594
NBP2-50061DL594
DyLight 650
NBP2-50061C
DyLight 680
NBP2-50061FR
DyLight 755
NBP2-50061IR
FITC
NBP2-50061F
HRP
NBP2-50061H
Janelia Fluor 525
NBP2-50061JF525
Janelia Fluor 549
NBP2-50061JF549
Janelia Fluor 585
NBP2-50061JF585
Janelia Fluor 635
NBP2-50061JF635
Janelia Fluor 646
NBP2-50061JF646
Janelia Fluor 669
NBP2-50061JF669
mFluor Violet 450 SE
NBP2-50061MFV450
mFluor Violet 500 SE
NBP2-50061MFV500
mFluor Violet 610 SE
NBP2-50061MFV610
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beta Tubulin Antibody (1B12) Summary

Immunogen
Pig brain beta Tubulin preparation. [UniProt# Q767L7]
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:5000
  • Single Cell Western
  • Western Blot 1:5000 - 1:10000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody (1B12)

  • beta polypeptide
  • beta Tubulin
  • MGC117247
  • OK/SW-cl.56
  • TUBB
  • TUBB5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-50061
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Species: Hu, Mu, Rt, Pm
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Bioinformatics

Gene Symbol TUBB
Uniprot