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beta I Tubulin Antibody (SR1866)

Images

 
Immunohistochemistry: beta I Tubulin Antibody (SR1866) [NBP3-21863] - Immunohistochemical analysis of paraffin-embedded human tonsil, using NBP3-21863.
Western Blot: beta I Tubulin Antibody (SR1866) [NBP3-21863] - Western blot analysis using NBP3-21863 in (1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (SR1866) [NBP3-21863] - Immunofluorescent analysis of Hela cells, using NBP3-21863.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SR1866
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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beta I Tubulin Antibody (SR1866) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human beta I Tubulin (Uniprot #: Q9H4B7)
Specificity
Detects endogenous levels of total beta I Tubulin
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TUBB1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Western Blot 1:3000-1:10000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for beta I Tubulin Antibody (SR1866)

  • beta tubulin 1, class VI
  • betaI Tubulin
  • beta-I Tubulin
  • dJ543J19.4
  • TUBB1
  • tubulin beta-1 chain
  • tubulin, beta 1

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta I Tubulin Antibody (NBP3-21863) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TUBB1