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beta Amyloid Antibody (MM32) [Unconjugated]

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Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181) coated on a Clear Polystyrene Microplate (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
MM32
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB96181-SP
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beta Amyloid Antibody (MM32) [Unconjugated] Summary

Immunogen
Recombinant protein to human Amyloid  beta aa35-40 peptide
Specificity
Detects human Amyloid  beta (aa1-40) in sandwich ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta Amyloid Antibody (MM32) [Unconjugated]

  • Abeta
  • Amyloid beta
  • beta-Amyloid Peptide

Background

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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