![Western Blot: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta-t2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Biotin-Western-Blot-NBP1-44521-img0001.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta-t2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line")
| Reactivity | Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)Species Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | B2M-01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Biotin |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NB110-60015 | |||
| HRP | NBP1-44520 | |||
| Unconjugated | NB500-317 | |||
| Immunogen | Purified human beta 2 Microglobulin (NM_004048.2). |
| Specificity | The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | B2M |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Readout System |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for beta 2-Microglobulin Antibody (NBP1-44521)Find related products by research area.
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| Gene Symbol | B2M |
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Human beta-2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control . Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Biotin-Immunocytochemistry-Immunofluorescence-NBP1-44521-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Human beta-2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control . Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Biotin-Flow-Cytometry-NBP1-44521-img0006.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) FITC.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Biotin-Flow-Cytometry-NBP1-44521-img0004.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) FITC.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Biotin-Flow-Cytometry-NBP1-44521-img0005.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [Biotin] [NBP1-44521] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) PerCP-CyTM5.5.")
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