![Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Western-Blot-NBP2-70242-img0004.jpg "Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.")
| Reactivity | Hu, Mu, CaSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI3F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCAT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for BCAT1 Antibody (NBP2-70242)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BCAT1 |
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0009.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.")
![Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Western-Blot-NBP2-70242-img0001.jpg "Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)")
![Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Western-Blot-NBP2-70242-img0002.jpg "Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)")
![Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Western-Blot-NBP2-70242-img0003.jpg "Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.")
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0005.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.")
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0006.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.")
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0007.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.")
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0008.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.")
![Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BCAT1-Antibody-OTI3F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70242-img0010.jpg "Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.")
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