![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0012.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: DSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BAG3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC reported in scientific literature (PMID: 30081850). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BAG3 Antibody (NBP1-86442)Find related products by research area.
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| Gene Symbol | BAG3 |
![Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86442-img0004.jpg "Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0015.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0013.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0014.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0016.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.")
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![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")