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B7-1/CD80 Antibody (2D10.4) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
2D10.4
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43384AF350
Alexa Fluor 405
NBP1-43384AF405
Alexa Fluor 488
NBP1-43384AF488
Alexa Fluor 532
NBP1-43384AF532
Alexa Fluor 594
NBP1-43384AF594
Alexa Fluor 647
NBP1-43384AF647
Alexa Fluor 700
NBP1-43384AF700
Alexa Fluor 750
NBP1-43384AF750
Allophycocyanin
NBP1-43384APC
Allophycocyanin/Cy7
NBP1-43384APCCY7
CoraFluor 1
NBP1-43384CL1
DyLight 350
NBP1-43384UV
DyLight 405
NBP1-43384V
DyLight 488
NBP1-43384G
DyLight 550
NBP1-43384R
DyLight 594
NBP1-43384DL594
DyLight 650
NBP1-43384C
DyLight 680
NBP1-43384FR
DyLight 755
NBP1-43384IR
FITC
NBP1-43384F
Janelia Fluor 525
NBP1-43384JF525
Janelia Fluor 549
NBP1-43384JF549
Janelia Fluor 646
NBP1-43384JF646
Janelia Fluor 669
NBP1-43384JF669
PE
NBP1-43384PE
PE/Atto594
NBP1-43384PEATT594
PE/Cy5.5
NBP1-43384PECY55
PE/Cy7
NBP1-43384PECY7
PerCP
NBP1-43384PCP
Unconjugated
NBP1-43384-0.1mg
mFluor Violet 450 SE
NBP1-43384MFV450
mFluor Violet 500 SE
NBP1-43384MFV500
mFluor Violet 610 SE
NBP1-43384MFV610
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B7-1/CD80 Antibody (2D10.4) [Biotin] Summary

Additional Information
Clone 2D10.4 was used by HLDA to establish CD designation.
Immunogen
The immunogen for this antibody was CD80.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (2D10.4) [Biotin]

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for B7-1/CD80 Antibody (NBP1-43616)

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Blogs on B7-1/CD80.

Check out the latest blog posts on B7-1/CD80.
CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.
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Bioinformatics

Gene Symbol CD80