![Western Blot: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Azide-and-BSA-Free-Western-Blot-NBP2-80587-img0001.jpg "Western Blot: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Rt, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 1F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Aurora A expressed in E. coli. [Uniprot: O14965] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AURKA |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Aurora A Antibody (NBP2-80587)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AURKA |
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80587-img0002.jpg "Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.")
![Flow Cytometry: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80587-img0003.jpg "Flow Cytometry: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.")
![ELISA: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Azide-and-BSA-Free-ELISA-NBP2-80587-img0004.jpg "ELISA: Aurora A Antibody (1F8) - Azide and BSA Free [NBP2-80587] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")