![Western Blot: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - ATPIF1 monoclonal antibody (M01), clone 3E2 Analysis of ATPIF1 expression in MCF-7.](http://images.novusbio.com/fullsize/ATPase-Inhibitory-Factor-1-Antibody-3E2-Western-Blot-H00093974-M01-img0008.jpg "Western Blot: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - ATPIF1 monoclonal antibody (M01), clone 3E2 Analysis of ATPIF1 expression in MCF-7.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | ATPIF1 (AAH09677, 1 a.a. ~ 106 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVTALAARTWLGVWGVRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD |
| Specificity | ATPIF1 - ATPase inhibitory factor 1 |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATP5IF1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Analysis of monoclonal antibody to ATPIF1 on MCF-7 cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATPase-Inhibitory-Factor-1-Antibody-3E2-Immunocytochemistry-Immunofluorescence-H00093974-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Analysis of monoclonal antibody to ATPIF1 on MCF-7 cell . Antibody concentration 10 ug/ml.")
![Western Blot: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Analysis of ATPIF1 expression in transfected 293T cell line by ATPIF1 monoclonal antibody (M01), clone 3E2.Lane 1: ATPIF1 transfected lysate(12 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ATPase-Inhibitory-Factor-1-Antibody-3E2-Western-Blot-H00093974-M01-img0009.jpg "Western Blot: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Analysis of ATPIF1 expression in transfected 293T cell line by ATPIF1 monoclonal antibody (M01), clone 3E2.Lane 1: ATPIF1 transfected lysate(12 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Detection limit for recombinant GST tagged ATPIF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATPase-Inhibitory-Factor-1-Antibody-3E2-Sandwich-ELISA-H00093974-M01-img0007.jpg "Sandwich ELISA: ATPase Inhibitory Factor 1 Antibody (3E2) [H00093974-M01] - Detection limit for recombinant GST tagged ATPIF1 is approximately 0.3ng/ml as a capture antibody.")
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