ATP6V1D Antibody

Images

 
Western Blot: ATP6V1D Antibody [NBP2-15518] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein at cytoplasm on Breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Breast carcinoma. ...read more
Western Blot: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein by Western blot analysis. A. 30 ug U87-MG whole cell lysate/extract 12 % SDS-PAGEV-ATPase D antibody dilution: 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Rb, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP6V1D Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human V-ATPase D. The exact sequence is proprietary.
Predicted Species
Rat (98%), Chicken (93%), Bovine (98%), Rabbit (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1D
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ATP6V1D Antibody

  • ATP6M
  • ATPase, H+ transporting lysosomal, member M
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump)
  • ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
  • H(+)-transporting two-sector ATPase, subunit M
  • vacuolar ATP synthase subunit D
  • vacuolar H-ATPase subunit D
  • vacuolar proton pump D subunit
  • vacuolar proton pump delta polypeptide
  • Vacuolar proton pump subunit D
  • vacuolar proton-ATPase subunit D
  • VATDV-ATPase D subunit
  • V-ATPase 28 kDa accessory protein
  • V-ATPase subunit D
  • VMA8
  • V-type proton ATPase subunit D

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol ATP6V1D
Uniprot