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ATP5F1 Antibody

Images

 
Independent Antibodies: Western Blot: ATP5F1 Antibody [NBP2-49255] - Analysis using Anti-ATP5F1 antibody NBP2-49255 (A) shows similar pattern to independent antibody NBP1-91689 (B).
Immunocytochemistry/ Immunofluorescence: ATP5F1 Antibody [NBP2-49255] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining of human heart muscle.
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining of human pancreas shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining in human kidney and pancreas tissues using anti-ATP5F1 antibody. Corresponding ATP5F1 RNA-seq data are presented for ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-ATP5F1 antibody NBP2-49255 (A) shows similar ...read more
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP2-49255] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATP5F1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LDYHISVQNMMRRKEQEHMINWVEKHVVQSISTQQEKETIAKCIADLKLLAKKAQAQPVM
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5PB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP5F1 Recombinant Protein Antigen (NBP2-49255PEP)

Reactivity Notes

Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP5F1 Antibody

  • ATP synthase B chain, mitochondrial
  • ATP synthase subunit b, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
  • ATPase subunit b
  • cell proliferation-inducing protein 47
  • H+-ATP synthase subunit b
  • MGC24431
  • PIG47

Background

ATP5F1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP5PB