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ATP Citrate Lyase Antibody (5F8D11) - BSA Free

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Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments ...read more
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.
Flow Cytometry: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.
ELISA: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
5F8D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37429-0.025ml
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ATP Citrate Lyase Antibody (5F8D11) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ATP Citrate Lyase (AA: 306-502) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACLY
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ATP Citrate Lyase Antibody (5F8D11) - BSA Free

  • ACLEC 2.3.3.8
  • ACLY
  • ATP Citrate Lyase
  • ATP citrate synthase
  • ATP-citrate (pro-S-)-lyase
  • ATP-citrate synthase
  • ATPCL
  • Citrate cleavage enzyme
  • CLATP
  • EC 2.3.3.8

Background

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACLY