Recombinant Human ASPA His Protein

Images

 
SDS-Page: Recombinant Human ASPA His Protein [NBP1-99096] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human ASPA His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-313 of Human ASPA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRA VKKCTRYIDC DLNRIFDLEN LGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSL APLPCYVYLI EHPSLKYATT RSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENG EIAAIIHPNL QDQDWKPLHP GDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ASPA
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
38.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-99096.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 M NaCl, 0.1 mM PMSF
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human ASPA His Protein

  • ACY-2
  • ACY2aminoacylase 2
  • Aminoacylase-2
  • ASPaminoacylase-2
  • aspartoacylase (aminoacylase 2, Canavan disease)
  • aspartoacylase
  • EC 3.5.1.15

Background

ASPA, also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, Aspartoacylase functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for ASPA Recombinant Protein (NBP1-99096)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-99096 Applications Species
Corti M, Byrne B, Gessler D et al. Case report of Adeno-Associated Virus-Mediated Gene Therapy in a Patient with Canavan Disease Using Simultaneous Dual Route of Administration and Immune Modulation Molecular Therapy - Methods & Clinical Development 2023-06-18

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Bioinformatics

Gene Symbol ASPA