![Western Blot: ARS2 Antibody [NBP2-42501] - Staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARS2-Antibody-Western-Blot-NBP2-42501-img0003.jpg "Western Blot: ARS2 Antibody [NBP2-42501] - Staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DPRAIVEYRDLDAPD corresponding to C-Terminus according to NP_001122324.1, NP_001122325.1, NP_001122326.1. |
| Specificity | This antibody is expected to recognize all reported isoform (NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1). |
| Predicted Species | Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SRRT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 140 kDa band observed in Daudi and in lysates of mouse spleen lysates (calculated MW of 101 kDa band according to NP_056992.4). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other sources. An additional band of unknown identity was also consistently observed at 37 kDa band in mouse spleen. This band was successfully blocked by incubation with the immunizing peptide. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-42501 | Applications | Species |
|---|---|---|
| Gruber JJ, Olejniczak SH, Yong J et al. Ars2 promotes proper replication-dependent histone mRNA 3' end formation. Mol Cell 2012-01-01 [PMID: 22244333] |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: ARS2 Antibody [NBP2-42501] - Analysis of Daudi (A) and Mouse Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARS2-Antibody-Western-Blot-NBP2-42501-img0001.jpg "Western Blot: ARS2 Antibody [NBP2-42501] - Analysis of Daudi (A) and Mouse Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
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