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Apolipoprotein E/ApoE Antibody (WUE-4) [FITC]

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Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [FITC] [NB110-60531F] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep24316), licensed under a CC-BY license.
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [FITC] [NB110-60531F] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto ...read more
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [FITC] [NB110-60531F] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
WUE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-60531AF350
Alexa Fluor 405
NB110-60531AF405
Alexa Fluor 488
NB110-60531AF488
Alexa Fluor 532
NB110-60531AF532
Alexa Fluor 594
NB110-60531AF594
Alexa Fluor 647
NB110-60531AF647
Alexa Fluor 700
NB110-60531AF700
Alexa Fluor 750
NB110-60531AF750
Allophycocyanin
NB110-60531APC
Biotin
NB110-60531B
CoraFluor 1
NB110-60531CL1
DyLight 350
NB110-60531UV
DyLight 405
NB110-60531V
DyLight 488
NB110-60531G
DyLight 550
NB110-60531R
DyLight 594
NB110-60531DL594
DyLight 650
NB110-60531C
DyLight 680
NB110-60531FR
DyLight 755
NB110-60531IR
HRP
NB110-60531H
Janelia Fluor 525
NB110-60531JF525
Janelia Fluor 549
NB110-60531JF549
Janelia Fluor 585
NB110-60531JF585
Janelia Fluor 635
NB110-60531JF635
Janelia Fluor 646
NB110-60531JF646
Janelia Fluor 669
NB110-60531JF669
PE
NB110-60531PE
PerCP
NB110-60531PCP
Unconjugated
NB110-60531
mFluor Violet 450 SE
NB110-60531MFV450
mFluor Violet 500 SE
NB110-60531MFV500
mFluor Violet 610 SE
NB110-60531MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB110-60531
BSA Free
NB110-60531SS
BSA Free
NB110-60531
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Apolipoprotein E/ApoE Antibody (WUE-4) [FITC] Summary

Immunogen
Purified human ApoE [UniProt# P02649]
Epitope
Residues 140-160 of purified human ApoE (LDLR receptor binding site).
Localization
Secreted
Specificity
This antibody detects ApoE2, ApoE3 and ApoE4 (PMID 2466929).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using
NB110-60531F in the following applications:

  • FLOW
    2 publications

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4) [FITC]

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein E/ApoE Antibody (NB110-60531F)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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Bioinformatics

Gene Symbol APOE