Apolipoprotein E/ApoE Antibody (1H4) Summary
Immunogen |
Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649] |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
APOE |
Purity |
Unpurified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:10000
- Flow Cytometry 1:200 - 1:400
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:200 - 1:1000
- Western Blot 1:500 - 1:2000
|
Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
Ascites |
Preservative |
0.03% Sodium Azide |
Purity |
Unpurified |
Alternate Names for Apolipoprotein E/ApoE Antibody (1H4)
Background
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for Apolipoprotein E/ApoE Antibody (NBP2-22110). (Showing 1 - 1 of 1 FAQ).
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Was the Flow Cytometry image which appears at the datasheet done on the HEPG2 cells by Intracellular staining with or without Golgi stop reagent?
- It was intracellular staining. We permeabilize the cells with 0.1% Triton x100 added for 15 min. Therefore, we anticipate that the "Golgi stop reagent" was not used, and since enough Apolipoprotein E was trapped in the cytoplasmic vesicles, like Golgi apparatus, at the time of fixation, and thus that reagent was not required to detect the protein target.
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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