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Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

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Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) [NBP2-77031] - Standard Curve Reference

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
28.12 ng/mL - 1800 ng/mL
Sensitivity
7 ng/mL

Order Details

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Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of Canine Apolipoprotein A-I/ApoA1. No significant cross-reactivity or interference between Canine Apolipoprotein A-I/ApoA1 and analogues was observed.
Standard Curve Range
28.12 ng/mL - 1800 ng/mL
Sensitivity
7 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
91 - 99%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOA1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay Plate
  3. HRP-Conjugate (100 x concentrate)
  4. HRP-Conjugate Diluent
  5. Instruction Manual
  6. Sample Diluent
  7. Standard (Freeze dried)
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (25 x concentrate)

Alternate Names for Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APOA1