Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of Canine Apolipoprotein A-I/ApoA1. No significant cross-reactivity or interference between Canine Apolipoprotein A-I/ApoA1 and analogues was observed. |
Standard Curve Range |
28.12 ng/mL - 1800 ng/mL |
Sensitivity |
7 ng/mL |
Assay Type |
Competitive ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Spike Recovery |
91 - 99% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
APOA1 |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate
- HRP-Conjugate (100 x concentrate)
- HRP-Conjugate Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Canine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)
Background
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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