![Western Blot: Annexin A1 Antibody [NBP1-33684] - Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-33684-img0013.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Pm, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. |
| Localization | Nucleus, Cytoplasm, Cell projection, cilium, Basolateral cell membrane |
| Predicted Species | Bovine (90%), Rabbit (91%), Primate (100%), Canine (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANXA1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Annexin A1 Antibody (NBP1-33684)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANXA1 |
![Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-33684-img0017.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody [NBP1-33684] - Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33684-img0014.jpg "Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody [NBP1-33684] - Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Western Blot: Annexin A1 Antibody [NBP1-33684] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-33684-img0015.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Paraffin-embedded N87 Xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-33684-img0005.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Paraffin-embedded N87 Xenograft, using antibody at 1:500 dilution.")
![Immunoprecipitation: Annexin A1 Antibody [NBP1-33684] - Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 ug of Annexin A1 antibody. Western blot analysis was performed using Annexin A1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunoprecipitation-NBP1-33684-img0010.jpg "Immunoprecipitation: Annexin A1 Antibody [NBP1-33684] - Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 ug of Annexin A1 antibody. Western blot analysis was performed using Annexin A1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.Annexin A1 stained by Annexin A1 antibody (NBP1-33684) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33684_rabbit-polyclonal-annexin-a1-antibody-91020242014679.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.Annexin A1 stained by Annexin A1 antibody (NBP1-33684) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (NBP1-33684) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33684_rabbit-polyclonal-annexin-a1-antibody-910202420122042.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (NBP1-33684) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Annexin A1 Antibody [NBP1-33684] - Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33684_rabbit-polyclonal-annexin-a1-antibody-161020241940572.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33684_rabbit-polyclonal-annexin-a1-antibody-1610202419414544.jpg "Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")