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alpha 1-Acid Glycoprotein Antibody [Unconjugated]

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Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a1-Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG ...read more
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3694-SP
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alpha 1-Acid Glycoprotein Antibody [Unconjugated] Summary

Immunogen
Human plasma-derived alpha 1-Acid Glycoprotein
Specificity
Detects human alpha 1-Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mose alpha 1-Acid Glycoprotein is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ORM1
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF3694 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 1-Acid Glycoprotein Antibody [Unconjugated]

  • AGP 1
  • AGP
  • AGP1
  • AGP1alpha-1-acid glycoprotein 1
  • AGPA
  • AGP-A
  • alpha 1Acid Glycoprotein
  • alpha 1-Acid Glycoprotein
  • OMD 1
  • ORM
  • ORM1
  • orosomucoid 1
  • Orosomucoid
  • orosomucoid-1

Background

alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive acute-phase reactant that is produced by the liver and secreted in plasma. Plasma alpha 1-acid concentration increases in response to inflammation and infection. alpha 1-acid glycoprotein belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. It is a 183 amino acid protein with 5 N-linked glycans that comprises 45% of the 43 kDa mass of the protein. The human and mouse protein share 43% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha 1-Acid Glycoprotein Antibody (AF3694)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ORM1