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AKT1 [p Thr308] Antibody (658320) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes or 100nM Calyculin A for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Akt1 (T308) Monoclonal Antibody (Catalog # MAB7419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Akt1 (T308) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 ...read more
Akt1 phosphorylated at T308 was detected in immersion fixed CCD-1070Sk human foreskin fibroblast cell line stimulated with Recombinant Human PDGF-BB (Catalog # 220-BB) using Mouse Anti-Human Phospho-Akt1 (T308) ...read more
ASPP2 blocks Gal-1 dependent nanoclustering and halts oncogenic H-ras induced transformation.Nanoclustering-FRET analysis in HEK cells coexpressing mGFP- and mCherry-tagged (A, B) H-rasG12V or (C, D) K-rasG12V. Cells ...read more
ASPP2 increases oncogenic H-ras, K-ras and N-ras-effector-recruitment, as well as ERK- and AKT-signalling.(A) Left, scheme explaining effector-recruitment FRET analysis in HEK cells. Right, examples of FLIM-FRET images ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
658320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7419-SP
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AKT1 [p Thr308] Antibody (658320) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human Akt1 T308 site
Modification
p Thr308
Specificity
Detects human and mouse Akt1 when phosphorylated at T308.
Source
N/A
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
AKT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB7419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT1 [p Thr308] Antibody (658320) [Unconjugated]

  • AKT serine/threonine kinase 1
  • AKT
  • Akt1
  • AKT1m
  • CWS6
  • EC 2.7.11
  • EC 2.7.11.1
  • PKB alpha
  • PKB
  • PKBMGC99656
  • PRKBA
  • Protein Kinase B Alpha
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • Serine-Threonine Protein Kinase
  • v-akt murine thymoma viral oncogene homolog 1
  • V-Akt Murine Thymoma Viral Oncogene-Like Protein 1

Background

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1. MAB7419 also detects mouse Phospho-Akt1 (T308) in Western Blot.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKT1 Antibody (MAB7419)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Simple Western, Western Blot.


Filter By Application
Simple Western
(1)
Western Blot
(2)
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Human
(2)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using MAB7419 Applications Species
B Koh, N Sulaiman, MB Fauzi, JX Law, MH Ng, TL Yuan, AGN Azurah, MH Mohd Yunus, RBH Idrus, MD Yazid A Three-Dimensional Xeno-Free Culture Condition for Wharton's Jelly-Mesenchymal Stem Cells: The Pros and Cons International Journal of Molecular Sciences, 2023-02-13;24(4):. 2023-02-13 [PMID: 36835154] (Simple Western, Human) Simple Western Human
Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer Breast Cancer Research 2020-12-09 [PMID: 33298139]
F Mirzamoham, A Kozlova, G Papaioanno, E Paltrinier, UM Ayturk, T Kobayashi Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models Nat Commun, 2018-04-10;9(1):1352. 2018-04-10 [PMID: 29636449] (Western Blot, Mouse) Western Blot Mouse
Itziar M. D. Posada, Benoit Lectez, Farid A. Siddiqui, Christina Oetken-Lindholm, Mukund Sharma, Daniel Abankwa Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1 Scientific Reports 2017-08-21 [PMID: 28827765]
Itziar M.D. Posada, Benoit Lectez, Mukund Sharma, Christina Oetken-Lindholm, Laxman Yetukuri, Yong Zhou et al. Rapalogs can promote cancer cell stemness in vitro in a Galectin-1 and H-ras-dependent manner Oncotarget 2017-07-04 [PMID: 28562352]
ASPP2 Is a Novel Pan-Ras Nanocluster Scaffold PLoS ONE, 2016-07-20;11(7):e0159677. 2016-07-20 [PMID: 27437940] (Western Blot, Human) Western Blot Human
Maja Šolman, Alessio Ligabue, Olga Blaževitš, Alok Jaiswal, Yong Zhou, Hong Liang et al. Specific cancer-associated mutations in the switch III region of Ras increase tumorigenicity by nanocluster augmentation eLife 2015-08-14 [PMID: 26274561]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AKT1 Antibody (MAB7419). (Showing 1 - 5 of 5 FAQs).

  1. How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?
    • Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
  2. Why are many of your antibodies formulated with sodium azide and BSA?
    • Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
  3. Do your HRP-conjugated antibodies contain sodium azide?
    • No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
  4. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
  5. What is the molecular weight of your antibodies?
    • All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).

Secondary Antibodies

 

Isotype Controls

Additional AKT1 Products

Blogs on AKT1. Showing 1-10 of 14 blog posts - Show all blog posts.

Check out the latest blog posts on AKT1.
Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.
Tips to Optimize Your Western Blot for Phosphorylated Protein Detection
By Jamshed Arslan, Pharm. D., PhD. Protein phosphorylation refers to a reversible post-translational modification in which a protein kinase adds a phosphate group to an amino acid residue of a target protein. Protein ...  Read full blog post.
Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.
Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.
Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.
AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by ...  Read full blog post.
Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.
AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis ...  Read full blog post.
Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.
Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.
Showing 1-10 of 14 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol AKT1