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Aiolos/IKZF3 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-24495AF350
Alexa Fluor 405
NBP2-24495AF405
Alexa Fluor 488
NBP2-24495AF488
Alexa Fluor 532
NBP2-24495AF532
Alexa Fluor 594
NBP2-24495AF594
Alexa Fluor 647
NBP2-24495AF647
Alexa Fluor 700
NBP2-24495AF700
Alexa Fluor 750
NBP2-24495AF750
Allophycocyanin
NBP2-24495APC
Biotin
NBP2-24495B
DyLight 350
NBP2-24495UV
DyLight 405
NBP2-24495V
DyLight 488
NBP2-24495G
DyLight 550
NBP2-24495R
DyLight 594
NBP2-24495DL594
DyLight 650
NBP2-24495C
DyLight 680
NBP2-24495FR
DyLight 755
NBP2-24495IR
FITC
NBP2-24495F
HRP
NBP2-24495H
Janelia Fluor 525
NBP2-24495JF525
Janelia Fluor 549
NBP2-24495JF549
Janelia Fluor 646
NBP2-24495JF646
Janelia Fluor 669
NBP2-24495JF669
PE
NBP2-24495PE
PerCP
NBP2-24495PCP
Unconjugated
NBP2-24495
mFluor Violet 450 SE
NBP2-24495MFV450
mFluor Violet 500 SE
NBP2-24495MFV500
mFluor Violet 610 SE
NBP2-24495MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-24495
BSA Free
NBP2-24495
BSA Free
NBP2-24495SS
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Aiolos/IKZF3 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A portion of amino acids 350-400 of human IKZF3 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IKZF3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: canine (88%), mouse (88%), rat (88%), equine (82%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Aiolos/IKZF3 Antibody [CoraFluor™ 1]

  • AIO
  • Aiolos
  • IKAROS family zinc finger 3 (Aiolos)
  • Ikaros family zinc finger protein 3
  • IKZF3
  • zinc finger protein Aiolos
  • ZNFN1A3
  • ZNFN1A3subfamily 1A, 3 (Aiolos)

Background

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IKZF3