![Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 over-expressed 293 cell line, cotransfected with CREB3L4 Validated Chimera RNAi ( Cat # H00148327-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3L4 monoclonal antibody (M01), clone 3E3 (Cat # H00148327-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Western-Blot-H00148327-M01-img0008.jpg "Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 over-expressed 293 cell line, cotransfected with CREB3L4 Validated Chimera RNAi ( Cat # H00148327-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3L4 monoclonal antibody (M01), clone 3E3 (Cat # H00148327-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF, KD |
| Clone | 3E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | CREB3L4 (NP_570968, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLGIPDLLDAWLEPPEDIFSTGSVLELGLHCPPPEVPVTRLQEQGLQGWKSGGDRGCGLQESEPEDFLKLFIDPNEVYCSEASPGSDSGISEDPCHPDSPPAPRATSSP |
| Specificity | CREB3L4 - cAMP responsive element binding protein 3-like 4 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CREB3L4 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for Western blot. It has also been used for RNAi validaton and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of monoclonal antibody to CREB3L4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Immunocytochemistry-Immunofluorescence-H00148327-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of monoclonal antibody to CREB3L4 on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 expression in transfected 293T cell line by CREB3L4 monoclonal antibody (M01), clone 3E3.Lane 1: CREB3L4 transfected lysate(43.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Western-Blot-H00148327-M01-img0009.jpg "Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 expression in transfected 293T cell line by CREB3L4 monoclonal antibody (M01), clone 3E3.Lane 1: CREB3L4 transfected lysate(43.4 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of protein-protein interactions between CREB3 and CREB3L4. HeLa cells were stained with anti-CREB3 rabbit purified polyclonal 1:1200 and anti-CREB3L4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIBZIP-Antibody-3E3-Proximity-Ligation-Assay-H00148327-M01-img0005.jpg "Proximity Ligation Assay: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of protein-protein interactions between CREB3 and CREB3L4. HeLa cells were stained with anti-CREB3 rabbit purified polyclonal 1:1200 and anti-CREB3L4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
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