Home » AG-2/AGR2 » AG-2/AGR2 Antibodies » AG-2/AGR2 Antibody (1C3)

AG-2/AGR2 Antibody (1C3)

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Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in MCF-7 (Cat # L046V1).
Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in MCF-7 (Cat # L046V1).
Western Blot: AG-2 Antibody (1C3) [H00010551-M03] - Analysis of AGR2 expression in transfected 293T cell line by AGR2 monoclonal antibody (M03), clone 1C3. Lane 1: AGR2 transfected lysatE (20 KDa). Lane 2: ...read more
ELISA: AG-2 Antibody (1C3) [H00010551-M03] - Detection limit for recombinant GST tagged AGR2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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AG-2/AGR2 Antibody (1C3) Summary

Immunogen
AGR2 (AAH15503.1, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKIPVSAFLLLVALSYTLARDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLLKTEL
Specificity
AGR2 - anterior gradient 2 homolog (Xenopus laevis)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AGR2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Publications
Read Publications using
H00010551-M03 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AG-2/AGR2 Antibody (1C3)

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

Anterior gradient homolog 2 (AGR2) is a proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. AGR2 is expressed in a number of different tumor types and high levels of AGR2 correlate with downregulation of the p53 response. AGR2 is being investigated for its use as a potential diagnostic and therapeutic target in various cancers, and AGR2 antibodies are important tools for cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AG-2/AGR2 Antibody (H00010551-M03)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using H00010551-M03 Applications Species
Eric C, F B, Stephanie B et al. AGR2 protein expression in colorectal tumour epithelialcompartment. Gut. 2022-12-09 [PMID: 36591613]
Amine M, Alice B, Cecile H et al. Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy-induced senescence escape. Int J Oncol. 2021-12-16 [PMID: 34913074]
Hana C, Petr V, Veronika K et al. AGR2-AGR3 hetero-oligomeric complexes: Identification and characterization. Bioelectrochemistry. 2021-03-26 [PMID: 33848875]
Delphine F, Claire B, Ines B et al. Extracellular AGR2 triggers lung tumour cell proliferation through repression of p21 CIP1. Biochim Biophys Acta Mol Cell Res. 2020-12-02 [PMID: 33278424]
Sommerova L, Ondrouskova E, Vojtesek B, Hrstka R et al. Suppression of AGR2 in a TGF-B-induced Smad regulatory pathway mediates epithelial-mesenchymal transition. BMC Cancer 2017-08-15 [PMID: 28810836]
Prochazkova I, Lenco J, Fucikova A et al. Targeted proteomics driven verification of biomarker candidates associated with breast cancer aggressiveness. Biochim Biophys Acta 2017-02-16 [PMID: 28216224]
Ahmad R, Nicora CD, Shukla AK et al. An efficient method for native protein purification in the selected range from prostate cancer tissue digests. Chin Clin Oncol 2016-12-02 [PMID: 28061542]
Dong A, Wodziak D, Lowe AW. EGFR Signaling Requires a Specific Endoplasmic Reticulum Thioredoxin for the Post-Translational Control of Receptor Presentation to the Cell Surface. J Biol Chem. 2015-02-09 [PMID: 25666625]
Riener MO, Thiesler T, Hellerbrand C et al. Loss of Anterior Gradient-2 expression is an independent prognostic factor in colorectal carcinomas. Eur J Cancer. 2014-04-30 [PMID: 24794000]
Gray TA, Murray E, Nowicki MW et al. Development of a fluorescent monoclonal antibody-based assay to measure the allosteric effects of synthetic peptides on self-oligomerization of AGR2 protein. Protein Sci. 2013-06-18 [PMID: 23780840]
Show All 16 Publications.

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Bioinformatics

Gene Symbol AGR2
Entrez
OMIM
Uniprot