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ADNP Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same ...read more
Western Blot: ADNP Antibody [NBP1-89236] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP1-89236] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.
Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.
Genetic Strategies: Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP1-89236) followed by ...read more
DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP1-89236) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ADNP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADNP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
  • Knockdown Validated Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ADNP Protein (NBP1-89236PEP)
Publications
Read Publications using
NBP1-89236 in the following applications:

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35052632).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ADNP Antibody

  • Activity-dependent neuroprotective protein
  • activity-dependent neuroprotector homeobox
  • activity-dependent neuroprotector
  • ADNP homeobox 1
  • ADNP
  • ADNP1
  • ADNP1KIAA0784activity-dependent neuroprotector homeobox protein

Background

The activity-dependent neuroprotective protein (hADNP) gene is frequently amplified in many neoplasias, including breast, bladder, ovarian, pancreatic, and colon cancers. hADNP mRNA is abundantly expressed in distinct normal tissues, and high expression levels were encountered in malignant cells. hADNP is implicated in maintaining cell survival, perhaps through modulation of p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ADNP Antibody (NBP1-89236)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-P, IP, Immunohistochemistry, WB, Western Blot.


Filter By Application
ICC/IF
(2)
IF/IHC
(3)
IHC-P
(1)
IP
(1)
Immunohistochemistry
(1)
WB
(2)
Western Blot
(1)
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Human
(2)
Mouse
(3)
Rat
(2)
Rabbit
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-89236 Applications Species
Magrì B, D'Amico AG, Maugeri G et al. Neuroprotective effect of the PACAP-ADNP axis on SOD1G93A mutant motor neuron death induced by trophic factors deprivation Neuropeptides 2023-10-11 [PMID: 37856900] (Immunohistochemistry, Western Blot, Mouse) Immunohistochemistry, Western Blot Mouse
D'Amico AG, Maugeri G, Magrì B et al. PACAP-ADNP axis prevents outer retinal barrier breakdown and choroidal neovascularization by interfering with VEGF secreted from retinal pigmented epitelium cells Peptides 2023-07-24 [PMID: 37495040] (WB, ICC/IF, IHC-P, Rat, Human) WB, ICC/IF, IHC-P Rat, Human
Ferreira ACF, Szeto ACH, Clark PA et al. Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation Immunity 2023-05-26 [PMID: 37285842] (IP, Mouse) IP Mouse
Gráinne M Donnelly, Isabel S Moore Sports Medicine and the Pelvic Floor. Current sports medicine reports 2023-03-06 [PMID: 36866951]
Maugeri G, D'Amico AG, Giunta S et al. Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium Antioxidants (Basel, Switzerland) 2022-01-07 [PMID: 35052632] (IF/IHC, WB, ICC/IF, Rabbit, Human) IF/IHC, WB, ICC/IF Rabbit, Human
Yang M, Chen Y, Tu S et al. Utilizing an Animal Model to Identify Brain Neurodegeneration-Related Biomarkers in Aging International Journal of Molecular Sciences 2021-03-23 [PMID: 33807010] (IF/IHC, Mouse) IF/IHC Mouse
Cesur G, Eren MK, Eren E et al. Effect of experimentally induced hypothyroidism during gestation period on activity dependent neurotrophic factor (ADNF) in newborn rat brain tissue. Horm Mol Biol Clin Investig 2018-09-26 [PMID: 30256757] (IF/IHC, Rat) IF/IHC Rat

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADNP