Orthogonal Strategies: Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same ...read more
Western Blot: ADNP Antibody [NBP1-89236] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP1-89236] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.
Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.
Genetic Strategies: Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP1-89236) followed by ...read more
DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP1-89236) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot ...read more
This antibody was developed against Recombinant Protein corresponding to amino acids: SGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADNP
Purity
Immunogen affinity purified
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Use in Rabbit reported in scientific literature (PMID:35052632).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for ADNP Antibody
Activity-dependent neuroprotective protein
activity-dependent neuroprotector homeobox
activity-dependent neuroprotector
ADNP homeobox 1
ADNP
ADNP1
ADNP1KIAA0784activity-dependent neuroprotector homeobox protein
Background
The activity-dependent neuroprotective protein (hADNP) gene is frequently amplified in many neoplasias, including breast, bladder, ovarian, pancreatic, and colon cancers. hADNP mRNA is abundantly expressed in distinct normal tissues, and high expression levels were encountered in malignant cells. hADNP is implicated in maintaining cell survival, perhaps through modulation of p53.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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