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ADAM10 Antibody (139712) [PE/Cy7]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
139712
Clonality
Monoclonal
Host
Rat
Conjugate
PE/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB946U-100UG
Alexa Fluor 405
FAB946V-100UG
Alexa Fluor 488
FAB946G-100UG
Alexa Fluor 532
FAB946X
Alexa Fluor 594
FAB946T-100UG
Alexa Fluor 647
FAB946R-100UG
Alexa Fluor 700
FAB946N
Alexa Fluor 750
FAB946S-100UG
Allophycocyanin
FAB946A-100UG
Allophycocyanin/Cy7
FAB946APCCY7
Biotin
FAB946B
CoraFluor 1
FAB946CL1
DyLight 350
FAB946D
DyLight 405
FAB946E
DyLight 488
FAB946K
DyLight 550
FAB946L
DyLight 594
FAB946M
DyLight 650
FAB946W
DyLight 680
FAB946Y
DyLight 755
FAB946Z
Fluorescein
FAB946F
HRP
FAB946H
Janelia Fluor 525
FAB946JF525
Janelia Fluor 549
FAB946I
Janelia Fluor 646
FAB946J
Janelia Fluor 669
FAB946JF669
PE/Atto594
FAB946PEATT594
PE/Cy5.5
FAB946PECY55
PerCP
FAB946C
Phycoerythrin
FAB946P-100UG
Unconjugated
MAB946-100
mFluor Violet 450 SE
FAB946MFV450
mFluor Violet 500 SE
FAB946MFV500
mFluor Violet 610 SE
FAB946MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB946-100
Carrier Free
FAB946F
Carrier Free
FAB946N
Carrier Free
MAB946-SP
Carrier Free
MAB946-500
Carrier Free
MAB946-100
Carrier Free
FAB946V-100UG
Carrier Free
FAB946U-100UG
Carrier Free
FAB946T-100UG
Carrier Free
FAB946S-100UG
Carrier Free
FAB946R-100UG
Carrier Free
FAB946G-100UG
Carrier Free
FAB946A-100UG
Carrier Free
FAB946P-100UG
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ADAM10 Antibody (139712) [PE/Cy7] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Thr215-Glu673
Accession # O35598
Specificity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ADAM10
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Alternate Names for ADAM10 Antibody (139712) [PE/Cy7]

  • a disintegrin and metalloprotease domain 10
  • a disintegrin and metalloproteinase domain 10
  • AD10
  • ADAM 10
  • ADAM metallopeptidase domain 10
  • ADAM10
  • CD156c antigen
  • CD156c
  • CDw156
  • disintegrin and metalloproteinase domain-containing protein 10
  • EC 3.4.24
  • HsT18717
  • kuz
  • Kuzbanian protein homolog
  • Kuzbanian
  • MADM
  • MADMEC 3.4.24.81
  • Mammalian disintegrin-metalloprotease

Background

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM10 Antibody
(139712) [PE/Cy7]
FAB946PECY7
ADAM10 Antibody (139712) [PE/Cy7]
Species: Mu
Applications: Flow

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Bioinformatics

Gene Symbol ADAM10