Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (optimum wavelength: 412 nm) Assay time: 20 min |
Standard Curve Range |
1.225 - 490 U/mL |
Sensitivity |
1.225 U/mL |
Inter-Assay |
%CV < 9.3 |
Intra-Assay |
%CV < 4.7 |
Spike Recovery |
Average recovery rate: 104% |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
ACHE |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Microplate
- Plate Sealer
- Reagent 1: Lysis Buffer
- Reagent 2: Buffer Solution
- Reagent 3: Chromogenic Agent
- Reagent 4: Substrate
|
Alternate Names for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)
Background
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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