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Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

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Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) [NBP3-24453] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma, animal tissue and cell samples
Standard Curve Range
1.225 - 490 U/mL
Sensitivity
1.225 U/mL

Order Details

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Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (optimum wavelength: 412 nm)
Assay time: 20 min
Standard Curve Range
1.225 - 490 U/mL
Sensitivity
1.225 U/mL
Inter-Assay
%CV < 9.3
Intra-Assay
%CV < 4.7
Spike Recovery
Average recovery rate: 104%
Kit Type
Activity Assay Kit (Colorimetric)
Gene
ACHE

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Microplate
  2. Plate Sealer
  3. Reagent 1: Lysis Buffer
  4. Reagent 2: Buffer Solution
  5. Reagent 3: Chromogenic Agent
  6. Reagent 4: Substrate

Alternate Names for Acetylcholinesterase/ACHE Activity Assay Kit (Colorimetric)

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ACHE