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Rat ACE/CD143 ELISA Kit (Colorimetric)

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ELISA: Rat ACE/CD143 ELISA Kit (Colorimetric) [NBP2-78732] - Samples were spiked with high concentrations of Rat ACE/CD143 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat ACE/CD143 ELISA Kit (Colorimetric) [NBP2-78732] - Samples were spiked with high concentrations of Rat ACE/CD143 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat ACE/CD143 ELISA Kit (Colorimetric) [NBP2-78732] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Rat ACE/CD143 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat ACE in samples. No Significant cross-reactivity or interference between Rat ACE and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.91%
Intra-Assay
CV% < 4.28%
Spike Recovery
87-111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ACE

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat ACE/CD143 ELISA Kit (Colorimetric)

  • ACE
  • ACE1angiotensin converting enzyme, somatic isoform
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
  • carboxycathepsin
  • CD143 antigen
  • CD143
  • DCP
  • DCP1
  • DCP1angiotensin-converting enzyme
  • dipeptidyl carboxypeptidase 1
  • Dipeptidyl carboxypeptidase I
  • EC 3.2.1.-
  • EC 3.4.15.1
  • Kininase II
  • MGC26566
  • MVCD3
  • peptidase P
  • testicular ECA

Background

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat ACE/CD143 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol ACE