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ACE/CD143 Antibody (ACE/3763)

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Western Blot: ACE/CD143 Antibody (ACE/3763) [NBP3-07608] - Western Blot Analysis of human Kidney tissue lysate using ACE/CD143 Mouse Monoclonal Antibody (ACE/3763).
Western Blot: ACE/CD143 Antibody (ACE/3763) [NBP3-07608] - Western Blot Analysis of human Kidney tissue lysate using ACE/CD143 Mouse Monoclonal Antibody (ACE/3763).
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3763) [NBP3-07608] - Formalin-fixed, paraffin-embedded human small intestine stained with ACE/CD143 Mouse Monoclonal Antibody (ACE/3763).
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3763) [NBP3-07608] - Formalin-fixed, paraffin-embedded human small intestine stained with ACE/CD143 Mouse Monoclonal Antibody (ACE/3763).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
ACE/3763
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08558AF350
Alexa Fluor 405
NBP3-08558AF405
Alexa Fluor 488
NBP3-08558AF488
Alexa Fluor 532
NBP3-08558AF532
Alexa Fluor 594
NBP3-08558AF594
Alexa Fluor 647
NBP3-08558AF647
Alexa Fluor 700
NBP3-08558AF700
Alexa Fluor 750
NBP3-08558AF750
Allophycocyanin
NBP3-08558APC
Biotin
NBP3-08558B
CoraFluor 1
NBP3-08558CL1
DyLight 350
NBP3-08558UV
DyLight 405
NBP3-08558V
DyLight 488
NBP3-08558G
DyLight 550
NBP3-08558R
DyLight 594
NBP3-08558DL594
DyLight 650
NBP3-08558C
DyLight 680
NBP3-08558FR
DyLight 755
NBP3-08558IR
FITC
NBP3-08558F
HRP
NBP3-08558H
Janelia Fluor 525
NBP3-08558JF525
Janelia Fluor 549
NBP3-08558JF549
Janelia Fluor 585
NBP3-08558JF585
Janelia Fluor 635
NBP3-08558JF635
Janelia Fluor 646
NBP3-08558JF646
Janelia Fluor 669
NBP3-08558JF669
PE
NBP3-08558PE
PerCP
NBP3-08558PCP
Unconjugated
NBP3-08558
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-08558
Azide and BSA Free
NBP3-08558
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ACE/CD143 Antibody (ACE/3763) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08558)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa 652-788) of human ACE/CD143 protein (Uniprot: P12821)
Localization
Cell Surface
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACE
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for ACE/CD143 Antibody (ACE/3763)

  • ACE
  • ACE1angiotensin converting enzyme, somatic isoform
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
  • carboxycathepsin
  • CD143 antigen
  • CD143
  • DCP
  • DCP1
  • DCP1angiotensin-converting enzyme
  • dipeptidyl carboxypeptidase 1
  • Dipeptidyl carboxypeptidase I
  • EC 3.2.1.-
  • EC 3.4.15.1
  • Kininase II
  • MGC26566
  • MVCD3
  • peptidase P
  • testicular ECA

Background

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACE
Uniprot