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ACAA1 Antibody (CL2650)

Images

 
Western Blot: ACAA1 Antibody (CL2650) [NBP2-42380] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251
Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2650) [NBP2-42380] - Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody, showing specific staining in peroxisomes in green. Microtubule- and ...read more
Immunohistochemistry: ACAA1 Antibody (CL2650) [NBP2-42380] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry: ACAA1 Antibody (CL2650) [NBP2-42380] - Staining of human colon shows granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry: ACAA1 Antibody (CL2650) [NBP2-42380] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry: ACAA1 Antibody (CL2650) [NBP2-42380] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL2650
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ACAA1 Antibody (CL2650) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQFLSDIPETVPLSTVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQ
Epitope
GAIMARIAQFLSDIP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACAA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Control Peptide
ACAA1 Recombinant Protein Antigen (NBP2-42380PEP)

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (88%), Rat (87%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for ACAA1 Antibody (CL2650)

  • acetyl-CoA acyltransferase 1
  • Acetyl-CoA acyltransferase
  • EC 2.3.1
  • peroxisomal

Background

Acetyl-Coenzyme A acyltransferase (ACAA1) is an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACAA1
Uniprot