![Western Blot: ABHD12 Antibody [NBP1-20875] - Analysis of ABHD12 in Mouse Brain lysate (35ug/ protein in RIPA buffer) Primary incubation was 1 hour Detected by chemiluminescence using NBP1-20875 at 0.3ug/ ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ABHD12-Antibody-Western-Blot-NBP1-20875-img0003.jpg "Western Blot: ABHD12 Antibody [NBP1-20875] - Analysis of ABHD12 in Mouse Brain lysate (35ug/ protein in RIPA buffer) Primary incubation was 1 hour Detected by chemiluminescence using NBP1-20875 at 0.3ug/ ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Mu, Rt, HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-REFLGKSEPEHQH corresponding to C-Terminus according to NP_056415.1. |
| Epitope | C terminus |
| Specificity | This antibody is expected to recognize isoform a (NP_001035937.1) only. |
| Predicted Species | Human (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ABHD12 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx. 40 kDa band observed in mouse brain and rat brain lysates (calculated MW of 45.1 kDa band according to human NP_001035937.1 and of 45.3 kDa band according to mouse NP_077785.2). |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
| Publication using NBP1-20875 | Applications | Species |
|---|---|---|
| Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol 2007-12-01 [PMID: 18096503] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ABHD12 |
| Entrez | |
| Uniprot |
|
