ABCG2/CD338 Antibody (5D3) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34^- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, Neutralization, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using MAB995	Applications	Species
S Ghosh, R Singh, ZM Vanwinkle, H Guo, PK Vemula, A Goel, B Haribabu, VR Jala Microbial metabolite restricts 5-fluorouracil-resistant colonic tumor progression by sensitizing drug transporters via regulation of FOXO3-FOXM1 axis Theranostics, 2022-07-18;12(12):5574-5595. 2022-07-18 [PMID: 35910798] (Western Blot, Human)	Western Blot	Human
M Ding, H Zhang, L Liu, R Liang Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells Oncol Lett, 2018-11-30;17(2):1531-1536. 2018-11-30 [PMID: 30675209] (Western Blot, Human)	Western Blot	Human
T Messmer, F von Meyenn, A Savino, F Santos, H Mohammed, ATL Lun, JC Marioni, W Reik Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Cell Rep, 2019-01-22;26(4):815-824.e4. 2019-01-22 [PMID: 30673604] (ICC, Human)	ICC	Human
K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling Stem Cell Reports, 2017-02-23;0(0):. 2017-02-23 [PMID: 28238797] (Flow Cytometry, Human)	Flow Cytometry	Human
Bhagwandin V, Bishop J, Wright W, Shay J The Metastatic Potential and Chemoresistance of Human Pancreatic Cancer Stem Cells. PLoS ONE, 2016-02-09;11(2):e0148807. 2016-02-09 [PMID: 26859746] (Neutralization, Human)	Neutralization	Human
Laura E. Sidney, Matthew J. Branch, Harminder S. Dua, Andrew Hopkinson Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells Cytotherapy 2015-12-01 [PMID: 26454751]
Guang Zhang, Zhongxia Wang, Weihuan Luo, Hongbo Jiao, Junhua Wu, Chunping Jiang Expression of Potential Cancer Stem Cell Marker ABCG2 is Associated with Malignant Behaviors of Hepatocellular Carcinoma Gastroenterology Research and Practice 2013-09-30 [PMID: 24194752]
Kobuchi , Hirotsug, Moriya , Koko, Ogino , Tetsuya, Fujita , Hirofumi, Inoue , Keiji, Shuin , Taro, Yasuda , Tatsuji, Utsumi , Kozo, Utsumi , Toshihik Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation. PLoS ONE, 2012-11-26;7(11):e50082. 2012-11-26 [PMID: 23189181] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Ji F, Wang Y, Sun H, Du J, Zhao H, Wang D, Xu Q, Duan D, Yang H Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression. J. Neuroimmunol., 2008-06-04;197(2):99-109. 2008-06-04 [PMID: 18534691] (ICC, Human)	ICC	Human
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X CD molecules 2006--human cell differentiation molecules. J. Immunol. Methods, 2006-12-04;319(1):1-5. 2006-12-04 [PMID: 17174972] (Flow Cytometry, Human)	Flow Cytometry	Human
Shimmura S, Nagoshi N, Matsuzaki Y, Okano H, Tsubota K Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea. Stem Cells, 2006-08-03;24(12):2714-22. 2006-08-03 [PMID: 16888282] (ICC, Mouse)	ICC	Mouse
Show All 11 Publications. Collapse Publications.

Array MAB995

• ABCG2/CD338 Antibodies
• ABCG2/CD338 ELISA Kits