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NLRP3/NALP3 Antibodies

NLRP3/NALP3 acts as a sensor component of the NLRP3 inflammasome and with the adapter, ASC/PYCARD, recruits proinflammatory caspases. Activation of the NALP3 inflammasome results in triggering proteolytic activity and secretion of cytokines in the interleukin-1 (IL-1) family. We offer antibodies to detect NLRP3 that have been cited in 105+ publications with 5+ customer reviews. Browse our extensive catalog for NLRP3 polyclonal antibodies, monoclonal antibodies, a recombinant rabbit monoclonal antibody, and NLRP3 conjugated antibodies. Validated research applications for NALP3 antibodies include CyTOF-ready, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, Flow Cytometry, IP, Simple Western, and Western Blot. Our NLRP3 antibodies can be used in a variety of model species including Human, Mouse, Rat, Bovine, Porcine, and Monkey. Choose the anti-NLRP3 antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each NLRP3 antibody is fully covered by our Guarantee+.

Alternate Names for NLRP3/NALP3 Antibodies

anti-NLRP3/NALP3 antibody, anti-NLRP3 antibody, anti-AII antibody, anti-AII/AVP antibody, anti-Angiotensin/vasopressin receptor AII/AVP-like antibody, anti-AVP antibody, anti-C1orf7 antibody, anti-Caterpiller protein 1.1 antibody, anti-CLR1.1 antibody, anti-Cold autoinflammatory syndrome 1 antibody, anti-Cold autoinflammatory syndrome 1 protein antibody, anti-Cryopyrin antibody, anti-Familial cold autoinflammatory syndrome antibody, anti-FCU antibody, anti-Leucine-rich repeat-, and PYD-containing protein 3 antibody, anti-Muckle-Wells syndrome antibody, anti-MWS antibody, anti-NACHT, LRR and PYD containing protein 3 antibody, anti-NACHT, LRR and PYD domains-containing protein 3 antibody, anti-NALP3 antibody, anti-NLR family, pyrin domain containing 3 antibody, anti-Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3 antibody, anti-PYPAF1 antibody, anti-PYRIN-containing APAF1-like protein 1 antibody

20 Results for "nlrp3-nalp3" in Primary Antibodies

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NBP2-12446

NLRP3/NALP3 Antibody - BSA Free

(4 Reviews)

(223 Publications)

0.2 ml

NBP2-12446

C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining and Western blot and expressed as the mean +/- SD. *p 0.05 versus the OA + Allopurinol group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29358971), licensed under a CC-BY licence.

Lycium barbarum polysaccharides lessened the changes in expression of pyroptosis markers (NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N)) in Abeta1-40 oligomers-exposed ARPE-19 cells. (A) Representative immunofluorescence images of NLRP3 (green fluorescence) and DAPI (blue fluorescence) in ARPE-19 cells under different treatments. Abeta1-40 oligomers exposure increased the cellular expressions of NLRP3 (second row). However, the increased expression was subsequently decreased by LBP treatment with both low (3.5 mg/L) and high (14 mg/L) concentration (third row and the fourth row). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32629957), licensed under a CC-BY licence.

Hippocampal AMPK/Sirt1 and NFkappaB/NLRP3/IL-1beta signaling pathways in diabetic rats and the effects of aerobic exercise. Type 2 diabetes significantly decreases the activation of hippocampal AMPK (a) and the level of Sirt1 (c), leading to increased Ac-NFkappaB (d) and NFkappaB (e); diabetic rats contain more NLRP3 inflammasomes (f) and IL-1beta (g). Aerobic exercise intervention significantly increases AMPK activity and Sirt1 concentration; aerobic exercise downregulates the Ac-NFkappaB and NFkappaB, leading to decreased levels of the NLRP3 inflammasome and IL-1beta. **p < 0.01, DM group vs. C group; #p < 0.05, TDM group vs. DM group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30911292), licensed under a CC-BY licence.

Exercise training prevents HFD-induced upregulation of cardiac pyroptosis. Western blotting was performed to determine the components of inflammasome complex and pyroptosis in the left ventricle of mice hearts. (A) HFD-fed obese mice show upregulation of the NLRP3 inflammasome in the left ventricle, which is prevented by exercise training. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31835893), licensed under a CC-BY licence.

NLRP3 deficiency in non-hematopoietic cells exacerbates GVHD and is not rescued by propionate. B6 WT and Gpr43-/- mice received BMT from either syngeneic B6 or allogeneic BALB/c donors. a) Representative immunoblots and densitometric analysis of NLRP3 normalized to the presence of beta-actin in IECs (CD326+) from allogeneic recipients 14 days after BMT (n = 3 each, two-tailed Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30201970), licensed under a CC-BY licence.

The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation factor 88 (MyD88), and nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON) or mechanical ventilation at high tidal volume (HTV) with saline, antimycin A (AmA) or cyclosporine A (CsA). (A) Levels of PINK1, Parkin, and Mfn1 mRNA. (B) Levels of LC3B, PINK1, Parkin, and Mfn1 protein by Western blot. (C) Relative expression of LC3B-II/LC3B-I and PINK1 protein. (D) Relative expression of Parkin and Mfn1 protein. (E) Levels of TLR9 and COX4 mRNA. (F) Levels of MyD88 and nuclear factor-kappa B (NF-kappa B) mRNA. (G) Levels of TLR9, COX4, MyD88, and NF-kappa B protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. (I) Relative expression of MyD88 and NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group;bP < 0.05 vs. HTV group; and cP < 0.05 vs. AmA group. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Cellular and embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K and CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin and CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin and CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) and submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 and Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium and in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Role of NF-kappa B in glucose fluctuation-induced inflammasome-related myocardial fibrosis. (A) Immunofluorescence staining of NF-kappa B/p65 nucleation (n = 3 per group). (B,C) The phosphorylation level of NF-kappa B/p65 in rat hearts of the three groups (n = 4 per group). (D) Immunofluorescence staining of intracellular NLRP3–ASC interaction in the GF and GF + TPCA-1 groups. The NRCFs were labeled with anti-NLRP3 (red), anti-ASC (green), and DAPI (blue) (n = 3 per group). (E–G) After application of TPCA-1 (0.5 μM), the protein expressions of cleaved caspase-1 and collagen I were measured by western blot (n = 3 per group). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, and p-IkB alpha in the hippocampus of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, and (E) p-IKB alpha in the hippocampus of the CCI-injured mice treated with oltipraz (OLT) or vehicle. The sham-operated mice (SHAM) treated with vehicle were used as controls. Representative examples of blots for (C) CD11b/c (160 kDa), PI3K (130 kDa), and GAPDH (37 kDa); and (F) for p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa), and IKB alpha (40 kDa). CD11b/c and PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 5 samples per experimental group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

LRRK2 regulation on alpha -synuclein levels. a Western blot analysis of basal alpha -synuclein and b p62 levels in neurons during mid and late differentiations. Blots show the alpha -synuclein soluble monomer of ~15 kDa. Bars represent the mean ± SEM of three to six values, including two different cell lines per group. beta III-tubulin was used as loading control (two-way ANOVA, ** P < 0.01, LRRK2G2019S v. control WT, late stage). cLRRK2 RNA levels 5 days after shRNA lentiviral transduction. The empty vector was used as the mock control. Lines represent the mean ± SEM of three to four independent silencing experiments. A representative immunoblot analysis of LRRK2 protein levels 5 days after transduction is also shown. beta III-tubulin was the loading control. d Immunoblots and corresponding quantification of alpha -synuclein 5 days after shLRRK2 transduction. Bars represent the mean ± SEM of at least three independent silencing experiments. LRRK2 silencing had a significant effect on alpha -synuclein protein levels (two-way ANOVA, *** P = 0.0002). Bonferroni post hoc test showed that this effect was limited to LRRK2 mutated mature neurons (**P < 0.05 for both LRRK2G2019S and LRRK2R1441G). e qRT-PCR analysis of SNCA after LRRK2 silencing. f Immunoblots and corresponding quantification of p62 5 days after shLRRK2 transduction. Bars represent the mean ± SEM of at least three independent silencing experiments. See Additional file 3 for uncropped blots Image collected and cropped by CiteAb from the following open publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells were subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium. (A) Vector control Caco-2 (VC) and OGR1 overexpressing Caco-2 cells where treated with tunicamycin at the indicated concentrations for 24 h. Total protein was isolated and Western blotting was performed. The results are representative of two independent experiments. (B) After 24 h pH shift, total protein was isolated and Western blotting was performed. The results are representative of three independent experiments. (C) Densitometry after normalization of BiP to beta -actin and (D) p-IRE1 alpha to total IRE1 alpha . Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). (E) After 24 h pH shift, total RNA was isolated and mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (*p < 0.05; **p < 0.01). (F) A specific small molecule OGR1 inhibitor (10 µM) was tested and the cells were subjected to low pH for 24 h, following 4–6 h incubation in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated and Western blot performed. Results are representative of two independent experiments. (G) Cells were treated as described in (F), then total RNA was extracted and analysed for expression of XBP1 (XBP1u) and spliced XBP1 (XBP1s) by conventional PCR. Results are representative of three independent experiments. (H) Quantification of the ratio of XBP1s/XBP1u was performed using ImageJ. Results are representative of two independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). For all the panels, the experiments were repeated two to three times. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified and gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V and CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left and right quadrants are platelets that are negative (94.0%) and positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) and 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, RM

Species Glossary

Reactivity: Hu, Mu, Rt, RM

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6668A

Alt Catalog# IMG-6668A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-12446AF350
Alexa Fluor 405	NBP2-12446AF405
Alexa Fluor 488	NBP2-12446AF488
Alexa Fluor 532	NBP2-12446AF532
Alexa Fluor 594	NBP2-12446AF594
Alexa Fluor 647	NBP2-12446AF647
Alexa Fluor 700	NBP2-12446AF700
Alexa Fluor 750	NBP2-12446AF750
Allophycocyanin	NBP2-12446APC
Biotin	NBP2-12446B
DyLight 350	NBP2-12446UV
DyLight 405	NBP2-12446V
DyLight 488	NBP2-12446G
DyLight 550	NBP2-12446R
DyLight 594	NBP2-12446DL594
DyLight 650	NBP2-12446C
DyLight 680	NBP2-12446FR
DyLight 755	NBP2-12446IR
FITC	NBP2-12446F
HRP	NBP2-12446H
Janelia Fluor 549	NBP2-12446JF549
Janelia Fluor 646	NBP2-12446JF646
PE	NBP2-12446PE
PerCP	NBP2-12446PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-12446SS

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MAB7578

NLRP3/NALP3 Antibody (768319) [Unconjugated]

(60 Publications)

100 μg

MAB7578

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates.

NLRP3/NALP3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

NLRP3/NALP3 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/ NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Human peripheral blood monocytes was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Sodium butyrate (NaB) and hydroxybutyric acid (HBA) eyedrops improve clinical parameters and decrease inflammation in animals subjected to alkali burns (AB). (A) Digital images of corneas stained with 0.1% sodium fluorescein demonstrating intact corneal epithelium after topical NaB, HBA or balanced salt solution (BSS) treatment for 5 days quater in die (QID) in untreated mice (n = 5 animals/group). Scale bar: 1000 µm; (B) Representative color digital images used to score corneal opacity (top row) and representative fluorescein stained corneas used to create wound closure rate (bottom row) at 5 days (n = 15 animals/group) after alkali burn. Scale bar: 1000 µm; (C) Corneal opacity in corneas subjected to alkali burn and topically treated with either NaB, HBA, or BSS and compared with dexamethasone (Dex) (n = 15 animals/group); (D) Wound closure rate in corneas subjected to alkali burn and topically treated with either NaB, HBA, or BSS and compared with Dex (n = 15 animals/group); (E) Mean ± SEM of results of gene expression analysis of NLRP3, Caspase-1, and IL-1 beta in whole cornea of animals subjected to alkali burn for 2 or 5 days and topically treated with either NaB, HBA, or BSS and compared with Dex (n = 5 animals/group). * p < 0.05, *** p < 0.001, **** p < 0.0001. (F) Representative digital images of western blot of NLRP3 and beta -actin in cornea epithelium of animals subjected to alkali burn for 2 days and topically treated with either NaB, HBA, or BSS (n = 12 animals/3 samples/group). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28273882), licensed under a CC-BY license. Not internally tested by R&D Systems.

Upregulation of nucleotide-binding oligomerization domain-containing protein (NOD)-like receptor family pyrin domain containing 3 (NLRP3) expression by corneal epithelium in animals subjected to alkali burn (AB) at 2 days (2 d) and 5 days (5 d) post-injury. (A) Gene expression analysis of NLRP3 messenger mRNA (mRNA) transcript in whole cornea of animals subjected to alkali burn (n = 8 animals/group). Graphs show means ± standard error of the mean (SEM). **** p < 0.0001; (B) Representative merged digital images of laser scanning confocal microscopy of corneas cryosections immunostained for NLRP3 (green) with propidium iodide nuclei counterstaining (DNA in red) in corneas subjected to alkali burn (images are representative of n = 6 animals/group). Scale bar: 50 µm; (C) Representative digital images of western blot of NLRP3 and beta -actin in cornea epithelium of animals subjected to alkali burn for 2 and 5 days. Each lane is a different sample (n = 12 animals/3 samples/group). MW: Molecular weight; UT: untreated control; 2 d and 5 d refer to mice subjected to alkali burn and euthanized 2 or 5 days post-injury, respectively (1 and 2 indicate different samples). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28273882), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC7578U-100UG
Alexa Fluor 405	IC7578V-100UG
Alexa Fluor 488	IC7578G
Alexa Fluor 532	FAB7578X
Alexa Fluor 594	IC7578T-100UG
Alexa Fluor 647	IC7578R-100UG
Alexa Fluor 700	IC7578N
Alexa Fluor 750	IC7578S-100UG
Allophycocyanin	IC7578A
Biotin	FAB7578B
DyLight 350	FAB7578D
DyLight 405	FAB7578E
DyLight 488	FAB7578K
DyLight 550	FAB7578L
DyLight 594	FAB7578M
DyLight 650	FAB7578W
DyLight 680	FAB7578Y
DyLight 755	FAB7578Z
FITC	FAB7578F
HRP	FAB7578H
Janelia Fluor 549	FAB7578I
Janelia Fluor 646	FAB7578J
PE	FAB7578P
Phycoerythrin	IC7578P
mFluor Violet 450 SE	FAB7578MFV450
mFluor Violet 500 SE	FAB7578MFV500
mFluor Violet 610 SE	FAB7578MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7578P
Carrier Free	IC7578A
Carrier Free	IC7578G
Carrier Free	IC7578N
Carrier Free	MAB7578-SP
Carrier Free	IC7578U-100UG
Carrier Free	IC7578T-100UG
Carrier Free	IC7578V-100UG
Carrier Free	IC7578S-100UG
Carrier Free	IC7578R-100UG

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NBP1-77080

NLRP3/NALP3 Antibody - BSA Free

(2 Reviews)

(30 Publications)

0.1 mg

NBP1-77080

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023/) licensed under a CC-BY license.

hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva and cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05.

IFI16 inhibits c-Myc-stimulated transcription and hTERT expression in HeLa cells.(A) Total protein extracts prepared from HeLa cells infected with control retrovirus (lane 1) or a virus encoding IFI16 protein (lane 2) were subjected to immunoblotting using antibodies specific to the indicated proteins. (B) Sub-confluent cultures of HeLa cells were transfected with pMyc-TA-luc reporter plasmid (1.0 µg) along with a second pRL-TK reporter plasmid (0.2 µg) and an empty plasmid (pCMV; column 1), a plasmid encoding c-Myc (column 2 and 4), a plasmid encoding IFI16 (column 3), or both plasmids encoding c-Myc and increasing amounts of the plasmid encoding IFI16 protein (column 5 and 6). After 44–48 h of transfections, cells were lysed and the lysates were analyzed for dual luciferase activity. Normalized relative luciferase activity in control cells is indicated as 1.0. (C) Total protein extracts prepared from HeLa cells infected with control retrovirus (lane 1) or a virus encoding antisense to IFI16 mRNA (lanes 2) were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ec

Species Glossary

Reactivity: Hu, Mu, Ec

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77080AF350
Alexa Fluor 405	NBP1-77080AF405
Alexa Fluor 488	NBP1-77080AF488
Alexa Fluor 532	NBP1-77080AF532
Alexa Fluor 594	NBP1-77080AF594
Alexa Fluor 647	NBP1-77080AF647
Alexa Fluor 700	NBP1-77080AF700
Alexa Fluor 750	NBP1-77080AF750
Allophycocyanin	NBP1-77080APC
Biotin	NBP1-77080B
DyLight 350	NBP1-77080UV
DyLight 405	NBP1-77080V
DyLight 488	NBP1-77080G
DyLight 550	NBP1-77080R
DyLight 594	NBP1-77080DL594
DyLight 650	NBP1-77080C
DyLight 680	NBP1-77080FR
DyLight 755	NBP1-77080IR
FITC	NBP1-77080F
HRP	NBP1-77080H
Janelia Fluor 549	NBP1-77080JF549
Janelia Fluor 646	NBP1-77080JF646
PE	NBP1-77080PE
PerCP	NBP1-77080PCP
mFluor Violet 450 SE	NBP1-77080MFV450
mFluor Violet 500 SE	NBP1-77080MFV500
mFluor Violet 610 SE	NBP1-77080MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77080SS

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NBP2-67639

NLRP3/NALP3 Antibody (SC06-23)

(4 Publications)

100 ul

NBP2-67639

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-90207

NLRP3/NALP3 Antibody

(6 Publications)

0.1 ml

NBP1-90207

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human spleen shows cytoplasmic positivity in lymphoid cells.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-97601

NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free

(6 Publications)

0.05 mg

NBP1-97601

SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 and H3 profiles shown as loading controls.(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA and enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red and blue, respectively.(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), and eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), and eRNA (2 kb from center) and across lincRNA gene bodies.(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.See also Figure S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-97601SS

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NBP2-03948

NLRP3/NALP3 Antibody (25N10E9) - BSA Free

(3 Publications)

0.1 mg

NBP2-03948

Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody in mouse NALP3 transfected cell lysate (A), human NALP3 transfected cell lysate (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6763A

Alt Catalog# IMG-6763A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-03948AF350
Alexa Fluor 405	NBP2-03948AF405
Alexa Fluor 488	NBP2-03948AF488
Alexa Fluor 532	NBP2-03948AF532
Alexa Fluor 594	NBP2-03948AF594
Alexa Fluor 647	NBP2-03948AF647
Alexa Fluor 700	NBP2-03948AF700
Alexa Fluor 750	NBP2-03948AF750
Biotin	NBP2-03948B
DyLight 350	NBP2-03948UV
DyLight 405	NBP2-03948V
DyLight 488	NBP2-03948G
DyLight 550	NBP2-03948R
DyLight 594	NBP2-03948DL594
DyLight 650	NBP2-03948C
DyLight 680	NBP2-03948FR
DyLight 755	NBP2-03948IR
FITC	NBP2-03948F
HRP	NBP2-03948H
Janelia Fluor 549	NBP2-03948JF549
Janelia Fluor 646	NBP2-03948JF646
mFluor Violet 450 SE	NBP2-03948MFV450
mFluor Violet 500 SE	NBP2-03948MFV500
mFluor Violet 610 SE	NBP2-03948MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-03948SS
Azide and BSA Free	NBP2-80881

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AF6789

NLRP3/NALP3 Antibody [Unconjugated]

(2 Publications)

100 μg

AF6789

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NALP1 or transfected with NLRP3/NALP3 and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 20 ng/mL PMA for 72 hours and 1 µg/mL LPS for 24 hours. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP3/NALP3 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

NLRP3/NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cells, unstimulated (lower panel) and stimulated (upper panel) with PMA and LPS, using Sheep Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for NLRP3/NALP3 at approximately 111 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human NLRP3/NALP3 (aa 4-223) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6789-SP

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MAB6789

NLRP3/NALP3 Antibody (652514)

(1 Publication)

100 μg

MAB6789

NLRP3/NALP3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6789-SP

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AF7010

NLRP3/NALP3 Antibody

(1 Publication)

100 μg

AF7010

NLRP3/NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line, unstimulated (lower panel) or stimulated (upper panel) with 50 ng/mL PMA and 200 ng/mL LPS, using Goat Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7010) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7010-SP

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MAB67892

NLRP3/NALP3 Antibody (652519) [Unconjugated]

100 μg

MAB67892

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC67892U-100UG
Alexa Fluor 350	FAB67892U
Alexa Fluor 405	IC67892V-100UG
Alexa Fluor 405	FAB67892V
Alexa Fluor 488	IC67892G-100UG
Alexa Fluor 488	FAB67892G
Alexa Fluor 532	FAB67892X
Alexa Fluor 594	IC67892T-100UG
Alexa Fluor 594	FAB67892T
Alexa Fluor 647	FAB67892R
Alexa Fluor 647	IC67892R-100UG
Alexa Fluor 700	FAB67892N
Alexa Fluor 700	IC67892N-100UG
Alexa Fluor 750	FAB67892S
Alexa Fluor 750	IC67892S-100UG
Allophycocyanin	FAB67892A
Biotin	FAB67892B
DyLight 350	FAB67892D
DyLight 405	FAB67892E
DyLight 488	FAB67892K
DyLight 550	FAB67892L
DyLight 594	FAB67892M
DyLight 650	FAB67892W
DyLight 680	FAB67892Y
DyLight 755	FAB67892Z
FITC	FAB67892F
HRP	FAB67892H
Janelia Fluor 549	FAB67892I
Janelia Fluor 646	FAB67892J
PE	FAB67892P
PerCP	FAB67892C
mFluor Violet 450 SE	FAB67892MFV450
mFluor Violet 500 SE	FAB67892MFV500
mFluor Violet 610 SE	FAB67892MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB67892-SP
Carrier Free	IC67892V-100UG
Carrier Free	IC67892U-100UG
Carrier Free	IC67892T-100UG
Carrier Free	IC67892S-100UG
Carrier Free	IC67892R-100UG
Carrier Free	IC67892N-100UG
Carrier Free	IC67892G-100UG

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MAB67891

NLRP3/NALP3 Antibody (447609) [Unconjugated]

100 μg

MAB67891

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 3 hours . PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB67891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NLRP3/NALP3 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB67891-SP

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page of 2

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

NLRP3/NALP3 Antibodies

NLRP3/NALP3 Antibody - BSA Free

NLRP3/NALP3 Antibody (768319) [Unconjugated]

NLRP3/NALP3 Antibody - BSA Free

NLRP3/NALP3 Antibody (SC06-23)

NLRP3/NALP3 Antibody

NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free

NLRP3/NALP3 Antibody (25N10E9) - BSA Free

NLRP3/NALP3 Antibody [Unconjugated]

NLRP3/NALP3 Antibody (652514)

NLRP3/NALP3 Antibody

NLRP3/NALP3 Antibody (652519) [Unconjugated]

NLRP3/NALP3 Antibody (447609) [Unconjugated]