NLRP3/NALP3 Antibodies
NLRP3/NALP3 acts as a sensor component of the NLRP3 inflammasome and with the adapter, ASC/PYCARD, recruits proinflammatory caspases. Activation of the NALP3 inflammasome results in triggering proteolytic activity and secretion of cytokines in the interleukin-1 (IL-1) family. We offer antibodies to detect NLRP3 that have been cited in 105+ publications with 5+ customer reviews. Browse our extensive catalog for NLRP3 polyclonal antibodies, monoclonal antibodies, a recombinant rabbit monoclonal antibody, and NLRP3 conjugated antibodies. Validated research applications for NALP3 antibodies include CyTOF-ready, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, Flow Cytometry, IP, Simple Western, and Western Blot. Our NLRP3 antibodies can be used in a variety of model species including Human, Mouse, Rat, Bovine, Porcine, and Monkey. Choose the anti-NLRP3 antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each NLRP3 antibody is fully covered by our Guarantee+.
Alternate Names for NLRP3/NALP3 Antibodies
anti-NLRP3/NALP3 antibody, anti-NLRP3 antibody, anti-AII antibody, anti-AII/AVP antibody, anti-Angiotensin/vasopressin receptor AII/AVP-like antibody, anti-AVP antibody, anti-C1orf7 antibody, anti-Caterpiller protein 1.1 antibody, anti-CLR1.1 antibody, anti-Cold autoinflammatory syndrome 1 antibody, anti-Cold autoinflammatory syndrome 1 protein antibody, anti-Cryopyrin antibody, anti-Familial cold autoinflammatory syndrome antibody, anti-FCU antibody, anti-Leucine-rich repeat-, and PYD-containing protein 3 antibody, anti-Muckle-Wells syndrome antibody, anti-MWS antibody, anti-NACHT, LRR and PYD containing protein 3 antibody, anti-NACHT, LRR and PYD domains-containing protein 3 antibody, anti-NALP3 antibody, anti-NLR family, pyrin domain containing 3 antibody, anti-Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3 antibody, anti-PYPAF1 antibody, anti-PYRIN-containing APAF1-like protein 1 antibody
20 Results for "nlrp3-nalp3" in Primary Antibodies
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Western blot analysis of TXNIP & NLRP3 protein expression in cultured HK-2 cells treated by normal glucose (5.5 mM), high glucose (30 mM), & NG + mannitol, respectively, for 72 hours, then following 4 hours of hypoxia & 2 hours of reoxygenation in HK-2 cells under high glucose stimulation with or without TXNIP siRNA & RES treatment, respectively. Representative blots (a) & quantitative analysis of Western blots for TXNIP (c) & NLRP3 (d), activity of caspase-1 (e), level of IL-1 beta (f), & Western blot of TXNIP gene knockdown in HK-2 cells (b). The data in (c–f) are means ± SE (n = 5). #P 0.05; *p 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-310202416113536.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Exercise training prevents HFD-induced upregulation of cardiac pyroptosis. Western blotting was performed to determine the components of inflammasome complex & pyroptosis in the left ventricle of mice hearts. (A) HFD-fed obese mice show upregulation of the NLRP3 inflammasome in the left ventricle, which is prevented by exercise training. (B) Apoptosis-associated speck adaptor protein (ASC) that assembles with NLRP3 & caspase-1 to make inflammasome complex is elevated in the heart of obese mice but attenuated by exercise training. (C) Exercise training prevents HFD-induced upregulation of cardiac caspase-1. (D) Expression of IL-1 beta , which is upregulated by HFD, is normalized by exercise training. All values are expressed as mean ± SEM with dots of different shape representing each animal in a group. One-way ANOVA & Tukey’s post-hoc test were used for statistical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31835893), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241612224.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 knockdown decreases corneal inflammation & suppressed neutrophil infiltration in mouse C. albicans keratitis. The C57BL/6 mice were subconjunctivally injected with 6 μL (4 × 108 PFU) of the Ad-GFP-shRNA or Ad-NLRP3-shRNA suspension 3 days before inoculation with 1 × 106 CFU C. albicans or with 5μL sterile PBS after the corneas were scratched. The mouse corneas or eyeballs were collected at 3 dpi & subjected for further detection. RT-qPCR analysis (A), western blot (B) & immunofluorescence staining (C) were used to verify the gene knockdown efficiency of Ad-NLRP3-shRNA (n = 3). Scale bar = 20 μm; magnification 400×. (D) Micrographs of Ad-GFP-shRNA & Ad-NLRP3-shRNA-pretreated mouse corneas were photographed at 3dpi. (E) Clinical score of the infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3-shRNA (n = 10). (F) Immunofluorescence staining was performed to assess the levels of neutrophils recruitment in mouse corneas after Ad-GFP-shRNA & Ad-NLRP3-shRNA pretreatment (n =3). Scale bar = 50 μm; magnification 200×. FK: fungal keratitis. All values are presented as mean ± SEM. **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241613619.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 knockdown decreases corneal inflammation & suppressed neutrophil infiltration in mouse C. albicans keratitis. The C57BL/6 mice were subconjunctivally injected with 6 μL (4 × 108 PFU) of the Ad-GFP-shRNA or Ad-NLRP3-shRNA suspension 3 days before inoculation with 1 × 106 CFU C. albicans or with 5μL sterile PBS after the corneas were scratched. The mouse corneas or eyeballs were collected at 3 dpi & subjected for further detection. RT-qPCR analysis (A), western blot (B) & immunofluorescence staining (C) were used to verify the gene knockdown efficiency of Ad-NLRP3-shRNA (n = 3). Scale bar = 20 μm; magnification 400×. (D) Micrographs of Ad-GFP-shRNA & Ad-NLRP3-shRNA-pretreated mouse corneas were photographed at 3dpi. (E) Clinical score of the infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3-shRNA (n = 10). (F) Immunofluorescence staining was performed to assess the levels of neutrophils recruitment in mouse corneas after Ad-GFP-shRNA & Ad-NLRP3-shRNA pretreatment (n =3). Scale bar = 50 μm; magnification 200×. FK: fungal keratitis. All values are presented as mean ± SEM. **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-310202416113526.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Heat-killed C. albicans (HKCA) activates NLRP3 inflammasome & induces pyroptosis in human corneal epithelial cells (HCECs). (A) The mRNA expression of NLRP3 in HCECs challenged with HKCA at an MOI of 1:500, 1:50, 1:5, 2:1, or 20:1 respectively for 4 hours was evaluated by RT-qPCR (n = 5). (B–D) The mRNA & protein expression of NLRP3 in HCECs exposed to HKCA (MOI = 20) for 0 (control), 2, 4, 8, 12, or 24 h (n = 3). (E) NLRP3 fluorescence intensity was evaluated using immunofluorescent staining for different times (12–36 h). (n = 3; Scale bar = 20 μm; magnification 400×). (F) Lactate dehydrogenase (LDH) of HCECs treated with HKCA (MOI = 20) for 24 h (n = 6). (G) The mRNA levels of ASC, CASP1, IL-1 beta , IL-18 & GSDMD in HCECs exposed to HKCA (MOI = 20) for different times (n = 3). (H,I) The protein expression of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) was examined by western blot (n = 3). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241611470.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis & apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, & cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, & cells without PA treatment served as the control. (C) Concentrations of IL-1 beta in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-beta , caspase-1, caspase-3, Bax, & Bcl-2. (E) The protein semiquantification is shown for P2X7R, NLRP3 & caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 & TGF-beta . (G) The protein semiquantification is shown for caspase-3, Bax, & Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, & then treated with 200 μM PA for 24 h. ∗p < 0.05 versus the CON group, #p < 0.05 versus the HFD group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31681001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241613631.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - STZ-induced diabetes induces TXNIP expression & NLRP3 inflammasome activation following I/R 48. TXNIP expression was examined by IHC (a), representative blots (b), & quantitative analysis of Western blots for TXNIP (c), NLRP3 (d) & pro-caspase-1 & cleaved caspase-1 (e-f), & release of IL-1 beta & IL-18 by ELISA (g-h). The data in (c–h) are means ± SE (n = 5). ★P < 0.05 versus NS group; #P < 0.05 versus DS group; ▲P < 0.05 versus NI/R group; &P < 0.05 versus DI/R group. NS & DS: nondiabetic & STZ-induced diabetic rats were subjected to sham operation. NI/R & DI/R: nondiabetic & STZ-induced diabetic rats were subjected to 25 min ischemia followed by 48 h reperfusion. DI/R-RES: STZ-induced diabetic rats that underwent I/R were treated with RES (10 mg/kg, ip daily) for 7 consecutive days before renal ischemia-reperfusion. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27867451), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-12446_rabbit-polyclonal-nlrp3-nalp3-antibody-310202416113521.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed mRNA (B) and protein (D) production of NLRP3. Data are shown as mean +/- SD. **P < 0.01 vs sham group. Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/137) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Western-Blot-NBP1-77080-img0011.jpg "Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed mRNA (B) and protein (D) production of NLRP3. Data are shown as mean +/- SD. **P < 0.01 vs sham group. Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/137) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0010.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-77080-img0003.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody---BSA-Free-Western-Blot-NBP1-77080-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NLRP3-NALP3 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal & conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva & cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-31020241535675.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of exogenous rHMGB1 significantly promoted the mRNA & protein production of NLRP3 (a, c) & ASC (e, g) & activation of caspase-1 (i). Thus, the intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed the production of NLRP3 (b, d) & ASC (f, h) & activation of caspase-1 (j). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415345338.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Co-immunoprecipitation assay: Figure showing interaction of NLRP3, NLRC4 & NAIP with ASC during CFT073 infection in THP-1m. Cytoplasmic extracts of THP-1m infected with or without CFT073, 6 h post stimulation were subjected to IP & WB by various antibodies (ASC, NLRP3, NLRC4, & NAIP) as described in material & methods. Cytoplasmic extract used as input was subjected to check NLRP3, ASC, NLRC4 & NAIP expression as shown in figure. ASC antibody was used to co-immunoprecipitate the complex from cytoplasmic extracts & then immunoblotted to detect NLRP3, NLRC4 & NAIP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415371971.jpg)
![Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-kappa B pathway. a, b NF-kappa B was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-kappa B p65. e, f Intravitreal injection of NF-kappa B p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 & decreased the processing of IL-1 beta , g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-kappa B p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77080_rabbit-polyclonal-nlrp3-nalp3-antibody-310202415291993.jpg)
![Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Western-Blot-NBP2-67639-img0007.jpg "Western Blot: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hr at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hrs.Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hr at room temperature.")
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of HUVEC cells labeling NLRP3/NAPL3 with Rabbit anti-NLRP3 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0011.jpg)
![Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Flow-Cytometry-NBP2-67639-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunocytochemistry-Immunofluorescence-NBP2-67639-img0006.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0004.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-SC06-23-Immunohistochemistry-Paraffin-NBP2-67639-img0010.jpg)
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0011.jpg "Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human tonsil shows moderate cytoplasmic positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0008.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0009.jpg)
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human spleen shows cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP1-90207-img0010.jpg)
![Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Simple-Western-NBP2-03948-img0004.jpg "Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis of Raw 264 cell lysate using NLRP3/NALP3 antibody (NBP2-03948) at 2 ug/ml.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Western-Blot-NBP2-03948-img0008.jpg)
![Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody in mouse NALP3 transfected cell lysate (A), human NALP3 transfected cell lysate (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Western-Blot-NBP2-03948-img0007.jpg)
