MCM2 Antibodies
We offer MCM2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each MCM2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MCM2 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat, Xenopus. Use the list below to choose the MCM2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MCM2 polyclonal antibodies and browse our MCM2 monoclonal antibody catalog.
Alternate Names for MCM2 Antibodies
anti-MCM2 antibody, anti-BM28MGC10606 antibody, anti-CCNL 1 antibody, anti-CCNL1 antibody, anti-CDCL1mitotin antibody, anti-EC 3.6.4.12 antibody, anti-KIAA0030MITOTIN antibody, anti-MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae) antibody, anti-minichromosome maintenance complex component 2 antibody, anti-minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin) antibody, anti-minichromosome maintenance deficient 2 (mitotin) antibody
27 Results for "mcm2" in Primary Antibodies
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0007.jpg "Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).")
![Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639] - Human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Immunohistochemistry-Paraffin-NB100-639-img0008.jpg)
![Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for 2 hours. Antibody: Affinity purified rabbit anti-phospho MCM2 (Ser108) antibody (Cat. No. NB100-639) used at 0.2 mcg/ml. Subsequently, total MCM2 was detected to verify loading. Detection: Chemiluminescence with an exposure time of 5 seconds.](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Whole cell lysate (15 or 50 ug) from 293T cells or mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibody used at 0.1 mcg/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0003.jpg)
![Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Western-Blot-NBP3-16066-img0009.jpg "Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0008.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunohistochemistry-Paraffin-NBP3-16066-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of PC-12 cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0007.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunohistochemistry-Paraffin-NBP3-16066-img0001.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded human tonsil using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunohistochemistry-Paraffin-NBP3-16066-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded mouse liver using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunohistochemistry-Paraffin-NBP3-16066-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded mouse lung using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunohistochemistry-Paraffin-NBP3-16066-img0004.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0029.jpg "Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Immunohistochemistry-NB100-2339-img0028.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0003.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0003.jpg "Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.")
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0006.jpg)
![Flow Cytometry: MCM2 Antibody (2B3) [NBP2-37566] - Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Flow-Cytometry-NBP2-37566-img0004.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0005.jpg)
![ELISA: MCM2 Antibody (2B3) [NBP2-37566] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-ELISA-NBP2-37566-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0003.jpg "Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.")
![Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by ICC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunocytochemistry-NBP3-07021-img0006.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0005.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji lysate. Antibody: Rabbit anti-Phospho-MCM2 recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) by WB of BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33953] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33953-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33953] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33953-img0005.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human pancreas shows low positivity in exocrine glanular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0012.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0013.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33954] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33954-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33954] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33954-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0009.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0017.jpg)
![Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Western-Blot-NB100-2569-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) used at a dilution of 1:200 (1 ug/ml).](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Immunohistochemistry-NB100-2569-img0032.jpg)
![Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml anti-phospho- MCM2 (pS40/41) in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Flow-Cytometry-NB100-2569-img0002.jpg)
![Western Blot: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 .](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Western-Blot-NBP2-45774-img0006.jpg "Western Blot: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 .")
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0005.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0001.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0003.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0004.jpg)