AHR Antibodies
We offer AHR Antibodies for use in common research applications: Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, RNA Inhibition, Sandwich ELISA, Simple Western, Western Blot. Each AHR Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AHR Antibodies can be used in a variety of model species: Guinea Pig, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the AHR Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AHR polyclonal antibodies and browse our AHR monoclonal antibody catalog.
Alternate Names for AHR Antibodies
anti-AHR antibody, anti-Ah receptor antibody, anti-AH-receptor antibody, anti-aryl hydrocarbon receptor antibody, anti-BHLHE76 antibody, anti-bHLHe76aromatic hydrocarbon receptor antibody, anti-Class E basic helix-loop-helix protein 76 antibody
12 Results for "ahr" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-128-img0004.jpg "Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-Paraffin-NB100-128-img0006.jpg)
![Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.](http://images.novusbio.com/fullsize/AHR-Antibody-Gel-Super-Shift-Assays-NB100-128-img0003.jpg)
![Knockdown Validated: AHR Antibody [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha. Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) and western blot (lower part). Relative expression level is shown as fold change normalized to scramble siRNA. All values represent the mean +/- SD of two independent experiments performed in three replicates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-58051-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AHR-Antibody-Knockdown-Validated-NB100-2289-img0014.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: AHR Antibody [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha. Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) and western blot (lower part). Relative expression level is shown as fold change normalized to scramble siRNA. All values represent the mean +/- SD of two independent experiments performed in three replicates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-58051-1), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-NB100-2289-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-AHR at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2289-img0012.jpg)
![Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.](http://images.novusbio.com/fullsize/AHR-Antibody-Western-Blot-NB100-2289-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HepG2 cells were fixed for 10 minutes using 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-AHR at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2289-img0015.jpg)
![Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U937 cells with NB100-2289 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/AHR-Antibody-Flow-Cytometry-NB100-2289-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2289-img0010.jpg)
![Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/AHR-Antibody-Flow-Cytometry-NB100-2289-img0013.jpg)
![Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AHR-Antibody-Simple-Western-NB100-2289-img0011.jpg)
![Western Blot: AHR Antibody - BSA Free [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha . (A) qPCR to detect mRNA level of CYP1A1, NQO1, HO1 & TRXR1 upon 20 h PFT-alpha treatment (20 µM) in MCF7 p53KO cells (upper part) & T47D cells (lower part). Relative expression level of CYP1A1 is shown in log2 scale; relative expression level of NQO1, HO1 & TRXR1 are shown as fold change, both normalized with DMSO treatment. All values represent the mean ± SD of two times independent experiments performed in three replicates. (B) Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) & western blot (lower part). Relative expression level is shown as fold change normalized to scramble siRNA. All values represent the mean ± SD of two independent experiments performed in three replicates. (C) DCF-DA staining of ROS levels upon doxorubicin treatment (1 µM, 8 h) with or without PFT-alpha (20 µM, 12 h pre-treatment) in MCF7 p53KO cells transfected with scramble siRNA & siAHR. For quantification of ROS levels (lower panel), the values are reported as percentage relative to DMSO treatment group. (D) qPCR to detect mRNA level of Nrf2 targets NQO1, HO1 & TRXR1 upon 20 h PFT-alpha treatment (20 µM) in MCF7 p53KO cells transfected with scramble siRNA & siAHR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31974452), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2289_rabbit-polyclonal-ahr-antibody-3102024165720.jpg)
![Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left lane A) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Western-Blot-NB300-515-img0018.jpg "Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left lane A) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in U251 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Immunocytochemistry-NB300-515-img0010.jpg)
![Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Flow-Cytometry-NB300-515-img0017.jpg)
![Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Western-Blot-NB300-515-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Immunocytochemistry-NB300-515-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in Hela Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Immunocytochemistry-NB300-515-img0009.jpg)
![Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Flow-Cytometry-NB300-515-img0012.jpg)
![Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in PC-3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/AHR-Antibody-RPT9-Flow-Cytometry-NB300-515-img0016.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0013.jpg "Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0010.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0012.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0009.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Normal biopsies of deparaffinized Human skeletal muscle tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0011.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0016.jpg "Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunocytochemistry-Immunofluorescence-H00000196-M02-img0012.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0008.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0014.jpg)
![Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunoprecipitation-H00000196-M02-img0013.jpg)
![ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-ELISA-H00000196-M02-img0015.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody [NBP1-89975] - Staining in human urinary bladder and pancreas tissues using anti-AHR antibody. Corresponding AHR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-Paraffin-NBP1-89975-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AHR Antibody [NBP1-89975] - Staining in human urinary bladder and pancreas tissues using anti-AHR antibody. Corresponding AHR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AHR Antibody [NBP1-89975] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-Paraffin-NBP1-89975-img0006.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody [NBP1-89975] - Staining of human urinary bladder shows high expression.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-Paraffin-NBP1-89975-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NBP1-89974] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89974-img0004.jpg "Immunocytochemistry/Immunofluorescence: AHR Antibody [NBP1-89974] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: AHR Antibody [NBP1-89974] - Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/AHR-Antibody-Immunohistochemistry-Paraffin-NBP1-89974-img0002.jpg)
![Western Blot: AHR Antibody (S07-1H9) [NBP3-19574] - Western blot detection of AHR in Hela cell lysates using NBP3-19574 (1:1000 diluted). Predicted band size: 96kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/AHR-Antibody-S07-1H9-Western-Blot-NBP3-19574-img0002.jpg "Western Blot: AHR Antibody (S07-1H9) [NBP3-19574] - Western blot detection of AHR in Hela cell lysates using NBP3-19574 (1:1000 diluted). Predicted band size: 96kDa. Observed band size: 100kDa.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (S07-1H9) [NBP3-19574] - Immunofluorescence of AHR (green) in Hela cells using NBP3-19574 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/AHR-Antibody-S07-1H9-Immunocytochemistry-Immunofluorescence-NBP3-19574-img0001.jpg)
![Western Blot: AHR Antibody (2Z5Y8) [NBP3-16312] - Western blot analysis of extracts of various cell lines, using AHR antibody (NBP3-16312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/AHR-Antibody-2Z5Y8-Western-Blot-NBP3-16312-img0002.jpg "Western Blot: AHR Antibody (2Z5Y8) [NBP3-16312] - Western blot analysis of extracts of various cell lines, using AHR antibody (NBP3-16312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")